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Etude et Imagerie de la polymerisation de l'actine

Abstract : The general topic of this work is the actin cytoskeleton. The dynamic polymeric arrangement is directly related to the cell morphology but also to the cell adhesion and motility. Its disruption is characteristic of cancerization, leading actin to be a pharmacological target.
Studying and imaging actin are the main goals of this work.
The first part of the manuscript deals with the development and characterization of a new in vitro screening test of actin polymerization. The polymerization is correlated with the enhancement of the fluorescence anisotropy of Alexa488 used as a tracer in cell extracts samples.
The second aspect is the development of a new FLIM (Fluorescence Lifetime Imaging Microscopy) and trFAIM (time-resolved Fluorescence Anistropy Imaging Microscopy) set-up. This set-up has been successfully used for actin imaging as well as for other topics developed in PPSM (organic nanocrystals, nanolatexs ...).
The last aspect exposes an original technology to study in vivo actin polymerization. After a femtosecond assisted nano-dissection, we were able to monitor the realignment of an actin stress fiber.
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Contributor : Jean-Alexis Spitz <>
Submitted on : Tuesday, October 2, 2007 - 10:49:35 AM
Last modification on : Monday, February 15, 2021 - 10:42:56 AM
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  • HAL Id : tel-00176019, version 1


Jean-Alexis Spitz. Etude et Imagerie de la polymerisation de l'actine. Autre. École normale supérieure de Cachan - ENS Cachan, 2006. Français. ⟨tel-00176019⟩



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