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Stem cells as gene delivery system to create cardiac biological pacemaker

Abstract : By investigating stem cell models, we showed that induction of oscillation in normal ventricular myocytes by connecting stem cells to them is not possible. Induction of oscillations by stem cells was never demonstrated yet in well controlled conditions. Reported results [5],[4] demonstrate only increase of oscillation frequency (in cell culture or in whole hearts).
Oscillations become possible only for myocytes with much lower than normal threshold for inducing oscillations. Approaches like decreasing of expression level of IK1 current in myocytes are successful.
Other possible approach is connecting stem cells not directly to myocytes but to other types of cardiac cells with lower oscillation threshold. This induces oscillation without need to affect IK1 current. This transitional cells might be AV node cells, Purkinje cells or cells in the vicinity of the sinus node.
To drive cardiac tissue, small size artificial pacemakers need deliver currents orders of magnitude larger than those used in cell pair or cell culture experiments. To avoid this, the size of the pacemaker created should be several times larger than the electrotonic constant ?
For pacemaker currents, most experimenters traditionally measure inactivation only. A description of a stem cell including inactivation only is not precise enough to study oscillations. Descriptions including both, activation and inactivation of pacemaker current should be used, contrary to tradition in this field.
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Contributor : Sandra Kanani <>
Submitted on : Saturday, September 29, 2007 - 10:42:24 AM
Last modification on : Wednesday, October 14, 2020 - 4:23:36 AM
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  • HAL Id : tel-00175655, version 1



Sandra Kanani. Stem cells as gene delivery system to create cardiac biological pacemaker. Physics [physics]. Université Nice Sophia Antipolis, 2005. English. ⟨tel-00175655⟩



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