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Analyse de la recombinaison des gènes TCRAD : réarrangements radio-induits et structure des jonctions signal.

Abstract : We have shown that irradiation of pre-TCR-deficient CD3ε-/- mice restores thymocyte differentiation, by a p53-dependent and by a p53-independent pathway. Events normally associated during normal thymocyte development are dissociated in response to radiation exposure. Both of these pathways require LAT expression. Therefore, radiation exposure activates pre-TCR-like signals. TCRA gene rearrangement is induced following radiation exposure. The signal joints resulting from TCRA gene rearrangement have the same structure than those found in wild type mice.
All signal joint analyzed in un-manipulated wild type mice do exhibit junctionnal diversity. This diversity results mainly from TdT activity. We present evidences that proteins involved in DNA repair and genomic stability participated in SJ formation. We propose that signal joint diversity is not an aberrant process but is a key feature of V(D)J recombination.
All our work increases our understanding of molecular events associated with V(D)J recombination.
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https://tel.archives-ouvertes.fr/tel-00175283
Contributor : Pascal Martinez <>
Submitted on : Thursday, September 27, 2007 - 3:03:33 PM
Last modification on : Friday, November 6, 2020 - 3:48:50 AM

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  • HAL Id : tel-00175283, version 1

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Cédric Touvrey. Analyse de la recombinaison des gènes TCRAD : réarrangements radio-induits et structure des jonctions signal.. Sciences du Vivant [q-bio]. Université Joseph-Fourier - Grenoble I, 2005. Français. ⟨tel-00175283⟩

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