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Formation de la condensation dermique chez l'embryon de poulet : études in vivo, in vitro et in silico

Abstract : The switch from embryonic homogeneous skin, composed by an epidermis overlying a dense dermis, to a heterogeneous skin, involving primordia of cutaneous appendages (epidermal placode and dermal condensation), is a complex phenomenon with morphological changes, which are regulated by several molecular factors. During my thesis, I have analyzed the molecular interactions required for dermal organization, before and during the dermal condensation formation.
The use of original in vitro methods, allowed me to identify molecular mechanisms involved in the arrest of cell proliferation, that is requested for dermal condensation formation and is under the control of p21WAF1CIP1 protein; cell migration, necessary for cell recruitment into follicular domain, that needs Integrins engagement with Fibronectin EIIIA isoform; chemotaxis via BMP-7 and arrested by BMP-2. I have also analyzed dermal condensation stabilization by Integrins and Notch pathway, which are both involved.
The collaboration with a mathematician allowed us to develop a mathematical model in silico integrating these previously studied parameters. Numerical simulations, by reproducing primordia formation in various conditions, are in accordance with my own biological results, but also explain older experiments from other laboratories, which have been previously mistaken.
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Theses
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https://tel.archives-ouvertes.fr/tel-00174691
Contributor : Frederic Michon <>
Submitted on : Tuesday, September 25, 2007 - 9:18:32 AM
Last modification on : Friday, November 6, 2020 - 3:45:53 AM
Long-term archiving on: : Friday, April 9, 2010 - 2:47:23 AM

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  • HAL Id : tel-00174691, version 1

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Frederic Michon. Formation de la condensation dermique chez l'embryon de poulet : études in vivo, in vitro et in silico. Biologie cellulaire. Université Joseph-Fourier - Grenoble I, 2007. Français. ⟨tel-00174691⟩

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