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Caractérisation des interactions glycosaminoglycannes/protéines dans le but de développer des molécules d'intérêt thérapeutique :
exemples de l'Endocan et de l'interféron gamma

Stéphane Sarrazin 1
1 LEM - Laboratoire d' Enzymologie Moléculaire
IBS - UMR 5075 - Institut de biologie structurale
Abstract : Proteoglycans are complex macromolecules which consist structurally of a core protein and one or more covalently linked glycosaminoglycan chains. Both components can interact with a variety of binding proteins known to be involved in various pathophysiological processes such as inflammation and cancer. The characterization of the specific interactions between glycosaminoglycans and their binding partners should raise new therapeutic opportunities in drug development.
In this context, we first studied a recently discovered proteoglycan called endocan. By developing new processes for high yield production and purification, we have been able to further characterise the structure of endocan using different biochemical approaches and also to study the interactions with various binding partners including interferon Γ and hepatocyte growth factor. In the same time, the characterisation of the specific interaction of interferon gamma and the particular glycosaminoglycan of heparan sulphate type allowed the synthesis by sugar chemistry of glycosaminoglycan mimetics. Among these molecules, a glycoconjugate modulates the activity of the cytokine and could lead to the development of a new type of drugs.
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https://tel.archives-ouvertes.fr/tel-00171170
Contributor : Stéphane Sarrazin <>
Submitted on : Tuesday, September 11, 2007 - 5:26:02 PM
Last modification on : Wednesday, November 4, 2020 - 3:03:14 PM
Long-term archiving on: : Friday, April 9, 2010 - 1:55:27 AM

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  • HAL Id : tel-00171170, version 1

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Stéphane Sarrazin. Caractérisation des interactions glycosaminoglycannes/protéines dans le but de développer des molécules d'intérêt thérapeutique :
exemples de l'Endocan et de l'interféron gamma. Biologie cellulaire. Université Joseph-Fourier - Grenoble I, 2007. Français. ⟨tel-00171170⟩

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