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DEVELOPPEMENTS EN IMAGERIE PAR
SPECTROMETRIE DE MASSE ET APPLICATIONS
AUX MODELES INVERTEBRES

Abstract : At the proteomics' time, the mass spectrometry appears to be a powerful tool to find
and identify peptides and proteins from purified sample. A new era is now beginning, with the
MALDI imaging, allowing both research, identification while conserving the localization of
biomolecules like peptides, proteins and lipids in tissues. Crucial developments at the level of
the sample preparations are in progress to improve the performances of this technology. In
this context, we were interested first of all in the settling of new protocols adapted to direct
analysis and Imaging mass spectrometry of small organisms in particular the leech Hirudo
medicinalis. This model is particularly interesting for the study of nervous regeneration and
we perfomed studies on lipids which can be implied in this physiological process. The second
point is the study of the contributions of metal coating for mass spectrometry. First of all a
metal evaporated on histological glass allows at the same time a correlation of the
morphological information with the molecular images of IMS. The coating of the sample,allowed preventing the mass shifts towards the highest m/z, to obtain best MALDI spectrum
and according to a more important reproducibility between 2 spectra, to produce MALDI
images with better qualities. Finally, a last part of the developments was dedicated to the
possibility of improving the image resolution by using a system allowing decreasing the
irradiated area by the laser.
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Submitted on : Saturday, August 18, 2007 - 4:34:59 PM
Last modification on : Thursday, February 21, 2019 - 10:33:55 AM
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M. Wisztorski. DEVELOPPEMENTS EN IMAGERIE PAR
SPECTROMETRIE DE MASSE ET APPLICATIONS
AUX MODELES INVERTEBRES. Sciences pharmaceutiques. Université des Sciences et Technologie de Lille - Lille I, 2006. Français. ⟨tel-00167308⟩

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