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Etude de la dynamique des adhésions neuronales N-cadhérine et L1 dans la croissance axonale et la synaptogenèse

Abstract : During the developmental process of axonal elongation and synaptogenesis, adhesion proteins such as cadherins and Ig-CAMs play a fundamental role by mediating neuronal contacts. In order to study the dynamics of these contacts and their role in these processes, we used imaging techniques : thrombin cleavage, FRAP, optical tweezers, quantum-dots, to observe live hippocampal primary neurons, which were associated to a semi-artificial system of microspheres coated with purified adhesion proteins (N-cadherin and L1). Using a L1 construct bearing an extracellular thrombin cleavable GFP, I could determine how the processes of diffusion and exo- endocytosis were involved in the dynamics of L1-dependant contacts and obtain quantitative data concerning L1 homophilic interaction. I also contributed to characterise the extracellular link between N-cadherin and GluR2, an AMPAR subunit, and the influence of N-cadherin expression on GluR2 mobility. The interaction between these two proteins could be implicated in the formation and/or the maturation of synapses.
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https://tel.archives-ouvertes.fr/tel-00164823
Contributor : Caroline Pruvost Née Dequidt <>
Submitted on : Monday, July 23, 2007 - 9:23:47 PM
Last modification on : Thursday, January 11, 2018 - 6:15:31 AM
Long-term archiving on: : Thursday, April 8, 2010 - 8:49:08 PM

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  • HAL Id : tel-00164823, version 1

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Caroline Pruvost Née Dequidt. Etude de la dynamique des adhésions neuronales N-cadhérine et L1 dans la croissance axonale et la synaptogenèse. Biophysique [physics.bio-ph]. Université Victor Segalen - Bordeaux II, 2007. Français. ⟨tel-00164823⟩

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