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Electrogreffage de biomolécules sur supports conducteurs pour le développement de biopuces à détection optique

Benjamin Corgier 1
1 LCO2 - Laboratoire de Chimie Organique 2 Glycochimie
MSMB - Méthodologie de synthèse et molécules bioactives
Abstract : This thesis presents screen-printed carbon microarray as immobilisation support for biochips. First, a model of enzymatic biochip based on an electrochemiluminescent detection and allowing the measuring of metabolites in serum samples is presented.
Then, an innovative immobilization strategy for biomolecules, leading to a covalent grafting on conductive microarrays is presented. This direct method, involving the electro-addressing properties of aryl-diazonium salts is characterized and used to electro-address antibodies, and to perform immuno-assays. As well, an oligonucleotide biochip is realized based on this model.
Gold electro-deposition at the surface of screen-printed electrodes, and its effects on biomolecule electro-addressing and on chemiluminescent detection are presented.
A third part presents the biomolecule electro-addressing on gold surfaces in order to realize SPR imaging detection.
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Submitted on : Monday, July 23, 2007 - 2:06:48 PM
Last modification on : Wednesday, November 20, 2019 - 2:41:11 AM
Long-term archiving on: : Thursday, April 8, 2010 - 8:47:23 PM

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Benjamin Corgier. Electrogreffage de biomolécules sur supports conducteurs pour le développement de biopuces à détection optique. Biochimie [q-bio.BM]. Université Claude Bernard - Lyon I, 2007. Français. ⟨tel-00164710⟩

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