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Etude des relations structure-fonction du transporteur mitochondrial d'ADP/ATP et de ses interactions avec d'autres protéines membranaires mitochondriales.

Abstract : Mitochondria are organelles found in every aerobic eukaryotic cell. They are surrounded by two membranes which compartmentalize essential physiological functions. The inner membrane forms a barrier that metabolites cannot cross without specific proteins such as the mitochondrial ADP/ATP carrier. The first part of this work deals with the purification of the bovine carrier complexed with bongkrekic acid. It follows on from previous studies carried out in our laboratory, whose aim was to solve the three-dimensional structure of the carrier locked in the different conformations involved in the nucleotide exchange. This complex appeared to dissociate during purification, so other strategies need to be designed to stabilize the complex before cristallisation trials. The two other parts of this work focus on two mitochondrial proteins which may interact functionally with the yeast Saccharomyces cerevisiae carrier. A membrane protein named yOm14, located in the mitochondrial external membrane, was characterized and its interaction with the carrier was clearly demonstrated. In addition, the yeast porin yVDAC1, which has previously been described as a carrier partner, was purified by setting up complementary methods detailed in this report. It will now be possible to carry out cristallisation trials, in order to solve the three-dimensional structure of the porin and to determine the molecular basis of its interaction with the ADP/ATP carrier.
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https://tel.archives-ouvertes.fr/tel-00162415
Contributor : Agathe Spira Afchain <>
Submitted on : Friday, July 13, 2007 - 2:06:29 PM
Last modification on : Friday, November 6, 2020 - 4:02:43 AM
Long-term archiving on: : Friday, November 25, 2016 - 6:26:29 PM

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  • HAL Id : tel-00162415, version 1

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Agathe Spira Afchain. Etude des relations structure-fonction du transporteur mitochondrial d'ADP/ATP et de ses interactions avec d'autres protéines membranaires mitochondriales.. Biochimie [q-bio.BM]. Université Joseph-Fourier - Grenoble I, 2007. Français. ⟨tel-00162415⟩

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