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LE NAADP, UN MESSAGER LIBERANT DU CALCIUM:
ETUDE DE L'IMPLICATION DES RESERVES ACIDES ET ROLE DE CD38 DANS LA VOIE DE SYNTHESE

Abstract : In pancreatic acinar cells, the secretagogues Acetylcholine (ACh) and cholecystokinin (CCK) trigger Ca2+ oscillations via the Ca2+ releasing second messengers IP3 and cADPR for ACh, and NAADP and cADPR for CCK. The existence of multiple messengers and multiple intracellular Ca2+ stores suggests a possible cooperation of these messengers and a selective recruitment of different Ca2+ stores.
Our work reveals that these Ca2+ oscillations evoked by CCK are initiated by Ca2+ release from the lysosome and endosome by NAADP. In the case of ACh stimulation, Ca2+ oscillations are initiated by Ca2+ release from the zymogen granule by IP3. The Ca2+ signals are then maintained and amplified by Ca2+ release from the endoplasmic reticulum (ER) under control of the cADPR.
We propose that the different Ca2+ releasing second messengers determine agonist-specific Ca2+ signatures by controlling the contribution of each acidic store and the ER.
Our data on NAADP synthesis show that the surface antigen CD38 is the main NAADP synthesizing enzyme in mice, as well as an other NAADP synthesizing enzyme that exists in brain. We also report first evidence that nitric oxyde is capable of inhibiting NAADP synthesis in skeletal muscle.
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https://tel.archives-ouvertes.fr/tel-00159587
Contributor : Alexis Menteyne <>
Submitted on : Tuesday, July 3, 2007 - 3:52:09 PM
Last modification on : Thursday, January 11, 2018 - 6:15:05 AM
Long-term archiving on: : Thursday, April 8, 2010 - 7:44:53 PM

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  • HAL Id : tel-00159587, version 1

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Alexis Menteyne. LE NAADP, UN MESSAGER LIBERANT DU CALCIUM:
ETUDE DE L'IMPLICATION DES RESERVES ACIDES ET ROLE DE CD38 DANS LA VOIE DE SYNTHESE. Biologie cellulaire. Université Paris Sud - Paris XI, 2007. Français. ⟨tel-00159587⟩

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