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Les cellules souches mésenchymateuses
du tissu adipeux humain :
Etude de leur potentiel myogénique

Abstract : Our laboratory has isolated from human infant adipose tissue, a mesenchymal stem cell population, named hMADS cells (human Multipotent Adipose-Derived Stem cells). hMADS cells exhibit at the single-cell level fundamental stem cell properties such self-renewal and multipotentiality.
In addition, mesenchymal stem cells may have an intrinsic capacity to differentiate into supplementary cell types in culture or after transplantation. Previously we have shown that hMADS cells were able to restore dystrophin expression after transplantation in mdx mice. In the current study, we investigated the mechanism of skeletal muscle phenotype acquisition by hMADS cells. We first tested various culture conditions to induce an intrinsic myogenic program in hMADS cells. Myotube formation and muscle determination/differentiation factors, i. e. Pax7, myf-5, MyoD and myogenin were assessed. No myogenic conversion could be detected under these conditions. In contrast, when co-cultured with myoblasts, hMADS cells reproducibly contributed to hybrid myotube formation, expressed several muscle differentiation markers and had the ability to restore dystrophin expression in human dystrophic background. Interestingly, this myogenic conversion was not associated by the expression of key transcription factors for muscle determination/differentiation.
Therefore, our results clearly demonstrated that acquisition of myogenic identity by hMADS cells did not occur via a bona fide determination/differentiation process. We propose a model of myogenic conversion of human mesenchymal stem cells derived from adipose tissue in which, only after fusion, transcription factors of myoblasts were able to program hMADS genome toward the skeletal muscle lineage.
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https://tel.archives-ouvertes.fr/tel-00156629
Contributor : Ari Massoudi <>
Submitted on : Thursday, June 21, 2007 - 7:30:57 PM
Last modification on : Wednesday, October 14, 2020 - 4:23:55 AM
Long-term archiving on: : Friday, November 25, 2016 - 3:51:22 PM

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  • HAL Id : tel-00156629, version 1

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Citation

Ari Massoudi. Les cellules souches mésenchymateuses
du tissu adipeux humain :
Etude de leur potentiel myogénique. Biologie cellulaire. Université Nice Sophia Antipolis, 2007. Français. ⟨tel-00156629⟩

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