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Dialogue entre le recepteur des oestrogènes alpha et le facteur de croissance IGF-I dans l'activation transcriptionnelle des cellules cancéreuses mammaires

Abstract : Insulin like growth factor I (IGF-I) displays estrogenic activity in breast cancer cells. This activity is strictly dependent on the presence of estrogen receptor Α (ERΑ). However the precise molecular mechanisms involved in this process are still unclear. IGF-I treatment induces phosphorylation of the AF1 domain of ERα and activation of estrogen regulated genes. These genes are characterized by important differences in promoter architecture and response element composition. We show that promoter structure is crucial for IGF-I induced transcription activation. We demonstrate that on a complex promoter such as the pS2/TFF1 promoter, which contains binding sites for ERα and for the AP1 complex, transcriptional activation by IGF-I requires both ERα and the AP1 complex. IGF-I is unable to stimulate transcription of an estrogen-regulated gene under the control of a minimal promoter containing only a binding site for ERα. We propose a molecular mechanism with stepwise assembly of the AP1 complex and ERα during transcription activation of pS2/TFF1 by IGF-I. IGF-I stimulation induces rapid phosphorylation and an increase in protein levels of the AP1 complex. Binding of the phosphorylated AP1 complex to the pS2/TFF1 promoter allows recruitment of the chromatin remodeling factor Brg1 followed by binding of ERα, via its interaction with c-Jun.
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https://tel.archives-ouvertes.fr/tel-00155908
Contributor : Sylvain Baron <>
Submitted on : Tuesday, June 19, 2007 - 2:32:21 PM
Last modification on : Wednesday, January 20, 2021 - 3:26:04 PM
Long-term archiving on: : Thursday, April 8, 2010 - 5:46:32 PM

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  • HAL Id : tel-00155908, version 1

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Sylvain Baron. Dialogue entre le recepteur des oestrogènes alpha et le facteur de croissance IGF-I dans l'activation transcriptionnelle des cellules cancéreuses mammaires. Biochimie [q-bio.BM]. Université Paul Sabatier - Toulouse III, 2007. Français. ⟨tel-00155908⟩

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