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Theses

ETUDE DE LA REGULATION DES RECEPTEURS DE PEPTIDES N-FORMYLES

Abstract : Phagocytes are the first host defense line against pathogens. Their directed migration to infection sites and their microbicidal functions result from intracellular signaling cascades elicited by specific G protein-coupled receptors, the chemoattractant receptors. After ligand binding and G protein-mediated signaling events, receptors are phosphorylated and interact with the b-arrestins, which are scaffolding proteins participating to receptor internalization. Recent examples suggest that b-arrestins could also participate to signaling events.
The work presented here is focused on the FPR family (Formyl Peptide Receptor) and specially the FPRL1 receptor (FPR-like 1), for which new agonists from bacterial origin or derived from human mitochondrial proteins have been identified. The phosphorylation of the FPRL1 receptor has been characterised and it has been shown that b-arrestins interact with this receptor and are essential to its internalization. Various approaches have concluded that the FPRL1-elicited rapid and transient activation of the MAP kinases ERK1/2 is mainly dependant on G protein and that there is no signaling events mediated by the b-arrestins. Finally, a proteomic study of the multiprotein complexes surrounded the couple FPRL1/b-arrestin has been conducted by the TAP method (Tandem Affinity Purification). The adaptor complex AP3, an homolog of AP2, has been identified as partner of b-arrestins after FPRL1 stimulation.
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https://tel.archives-ouvertes.fr/tel-00154576
Contributor : Emilie Huet Moulard <>
Submitted on : Thursday, June 14, 2007 - 10:42:42 AM
Last modification on : Friday, November 6, 2020 - 4:11:14 AM
Long-term archiving on: : Friday, September 21, 2012 - 4:30:33 PM

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  • HAL Id : tel-00154576, version 1

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Emilie Huet Moulard. ETUDE DE LA REGULATION DES RECEPTEURS DE PEPTIDES N-FORMYLES. Biologie cellulaire. Université Joseph-Fourier - Grenoble I, 2007. Français. ⟨tel-00154576⟩

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