Un microlaboratoire électrophorétique pour l'étude
du couplage entre transport et cinétique chimique :
application à la réaction d'hybridation d'oligonucléotides

Abstract : We have fabricated a microlaboratory to study diffusion and its coupling with a chemical
reaction. It is composed by an analysis chamber in which electric fields in two
dimensions can be applied to yield stationary and non-stationary concentration profiles.
The spatial simplicity of the electrophoretic flux used allows to systematically analyze
data in the Fourier space. This treatment yields diffusion coefficients of a pure analyte
and thermodynamic and kinetic constants of a reacting mixture. In the scope of developing
separation techniques based on kinetics, we have studied the hybridization reaction
between DNA oligonucleotides with a wide range of kinetic constants. These studies
made us to develop, in collaboration with the Museum National d'Histoire Naturelle, a
quadruplex-based molecular beacon that allows a kinetic discrimination when it hybridizes
to a matched or a mismatched sequence.
Document type :
Theses
Autre. Université Pierre et Marie Curie - Paris VI, 2007. Français


https://tel.archives-ouvertes.fr/tel-00153416
Contributor : André Estévez-Torres <>
Submitted on : Wednesday, July 11, 2007 - 3:54:45 PM
Last modification on : Saturday, December 5, 2015 - 1:34:50 AM
Document(s) archivé(s) le : Tuesday, September 21, 2010 - 1:17:38 PM

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  • HAL Id : tel-00153416, version 2

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André Estévez-Torres. Un microlaboratoire électrophorétique pour l'étude
du couplage entre transport et cinétique chimique :
application à la réaction d'hybridation d'oligonucléotides. Autre. Université Pierre et Marie Curie - Paris VI, 2007. Français. <tel-00153416v2>

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