Un microlaboratoire électrophorétique pour l'étude
du couplage entre transport et cinétique chimique :
application à la réaction d'hybridation d'oligonucléotides

Abstract : We have fabricated a microlaboratory to study diffusion and its coupling with a chemical
reaction. It is composed by an analysis chamber in which electric fields in two
dimensions can be applied to yield stationary and non-stationary concentration profiles.
The spatial simplicity of the electrophoretic flux used allows to systematically analyze
data in the Fourier space. This treatment yields diffusion coefficients of a pure analyte
and thermodynamic and kinetic constants of a reacting mixture. In the scope of developing
separation techniques based on kinetics, we have studied the hybridization reaction
between DNA oligonucleotides with a wide range of kinetic constants. These studies
made us to develop, in collaboration with the Museum National d'Histoire Naturelle, a
quadruplex-based molecular beacon that allows a kinetic discrimination when it hybridizes
to a matched or a mismatched sequence.
Document type :
Theses
Other. Université Pierre et Marie Curie - Paris VI, 2007. French


https://tel.archives-ouvertes.fr/tel-00153416
Contributor : André Estévez-Torres <>
Submitted on : Wednesday, July 11, 2007 - 3:54:45 PM
Last modification on : Thursday, July 12, 2007 - 2:36:34 PM

Identifiers

  • HAL Id : tel-00153416, version 2

Citation

André Estévez-Torres. Un microlaboratoire électrophorétique pour l'étude
du couplage entre transport et cinétique chimique :
application à la réaction d'hybridation d'oligonucléotides. Other. Université Pierre et Marie Curie - Paris VI, 2007. French. <tel-00153416v2>

Export

Share

Metrics

Consultation de
la notice

119

Téléchargement du document

199