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. Dans-sa-forme-habituelle and . La-spectroscopie-À-corrélation-de-fluorescence, Fluorescence Correlation Spectroscopy ou FCS), consiste à enregistrer pendant quelques dizaines de secondes la fluorescence émise par un petit nombre de molécules diffusant dans un petit volume de collection (? 0,1 µm 3 ) situé au foyer de l'objectif de grande ouverture numérique d'un microscope confocal. La fonction d'autocorrélation du signal correspondant est directement reliée au nombre moyen de molécules observées et à leur constante de diffusion, Les applications de cette technique concernent la biologie cellulaire et la biochimie, avec comme objectif de mesurer les concentrations de molécules de tailles différentes et leurs interactions

. La-dernière-géométrie-fait-intervenir-les-modulateurs-spatiaux-de-lumière, Spatial Light Modulator ou SLM) Ces instruments modifient localement le front d'onde d'une illumination, ce qui permet, au foyer d'un objectif de créer une grande variété d'illuminations