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PHYSIOPATHOLOGIE DE L'ARTHROSE : RÔLE DE LA NADPH OXYDASE NOX 4 DANS L'EXPRESSION
DE LA COLLAGÉNASE, MMP-1.

Laurent Grange 1
1 GREPI
TIMC - Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525
Abstract : Reactive Oxygen Species (ROS) are regulators of redox-sensitive cell-signaling pathways. In osteoarthritis, human interleukin-1β is implicated in cartilage destruction through an ROS dependent matrix metalloproteinase production. To determine the molecular source of ROS production in the human IL-1β (hIL-1β)-sensitive chondrocyte immortalized cell line C-20/A4, we constructed transfected cells that overexpress NAD(P)H oxidases. Firstly, RT-PCR analysis showed that the C-20/A4 cell line expressed Nox2, Nox4, p22phox and p67phox. It was found that ROS production by C-20/A4 chondrocytes does not depend on PMA and ionomycin activation. This indicates that Nox2 was not involved in the production of ROS. In C-20/A4 cells which overexpress Nox4, hIL-1β stimulated ROS production three times more than the normal production of C-20/A4 cells. Moreover, there was a fourfold increase in the production of collagenase (MMP-1) by chondrocytes which overexpress Nox4. Interestingly, MMP-1 production in cells which overexpress Nox2 was not sensitive to hIL-1β. Our data suggest that under hIL-1β stimulation, C-20/A4 chondrocytes produce MMP-1 through a Nox4-mediated, ROS dependent pathway.
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https://tel.archives-ouvertes.fr/tel-00148919
Contributor : Laurent Grange <>
Submitted on : Wednesday, May 23, 2007 - 5:12:31 PM
Last modification on : Thursday, November 19, 2020 - 3:56:07 PM
Long-term archiving on: : Thursday, April 8, 2010 - 5:39:59 PM

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  • HAL Id : tel-00148919, version 1

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Laurent Grange. PHYSIOPATHOLOGIE DE L'ARTHROSE : RÔLE DE LA NADPH OXYDASE NOX 4 DANS L'EXPRESSION
DE LA COLLAGÉNASE, MMP-1.. Biochimie [q-bio.BM]. Université Joseph-Fourier - Grenoble I, 2007. Français. ⟨tel-00148919⟩

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