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Theses

La lectine PA-IIL de Pseudomonas aeruginosa :
Structure, affinité et spécificité pour des ligands
naturels et glycomimétiques

Abstract : The chronic colonization of the lungs by P. aeruginosa is a major cause of infection and
mortality among cystic fibrosis patients (CF). For the pathogenic bacterium, the ability to
adhere to the host cells is essential to initiate the infection, and the glycoconjugates of the
surface of the epithelial cells are as many targets for the bacterial receptors. Among CF
patients, modifications occur in glycoconjugates of the epithelial cell surface on pulmonary
and salivary mucins. An increase in fucosylated oligosaccharides of Lewis type is observed,
often with sialylation and/or sulfatation. A soluble lectin, PA-IIL, recognizing the fucose
specifically, was identified in the cytoplasm of P. aeruginosa. This lectin, also found on the
surface of the bacterium, could be implied in the recognition of the host and/or the formation
of the biofilm that is responsible for the resistance of the bacterium against antibiotics. The
work developed in the thesis concerns the structural and thermodynamic study of the
interaction of PA-IIL with natural ligands and glycomimétics. Tests of interaction ELLA
(Enzyme-Linked Lectin Assays) were set up in order to evaluate the properties of inhibition
of these compounds. This work constitutes a beginning in the design of new compounds
derived from sugars. Theses glycomimetics could be use as anti-bacterial in the infections
occurring among CF patients. (Project financed by association: Vaincre la Mucoviscidose)
Document type :
Theses
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https://tel.archives-ouvertes.fr/tel-00144113
Contributor : Charles Sabin <>
Submitted on : Monday, April 30, 2007 - 5:28:41 PM
Last modification on : Tuesday, November 3, 2020 - 9:24:07 AM
Long-term archiving on: : Tuesday, April 6, 2010 - 11:07:31 PM

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  • HAL Id : tel-00144113, version 1

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Charles Sabin. La lectine PA-IIL de Pseudomonas aeruginosa :
Structure, affinité et spécificité pour des ligands
naturels et glycomimétiques. Médicaments. Université Joseph-Fourier - Grenoble I, 2006. Français. ⟨tel-00144113v1⟩

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