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Développement d'interfaces adaptées aux analyses biochimiques et biologiques. Application aux capteurs chimiques CHEMFETs

Abstract : Medical analysis techniques field needs low cost development of reliable chemical sensors. In this context, chemical field effect transistors ChemFET provide innovating answers but this will require microtechnology/ biotechnology interface optimization. In this work, we set out to develop techniques which allow us to link biologic species and silicon material. We studied two kinds of approaches both based on polymer use. First approach aimed improvement of encapsulation techniques micro-volumes creation. In this perspective, micro-tanks were fabricated, first in plexiglass® and secondly, in PDMS. Following optimization of geometric characteristics, bacterial activity was monitored thanks to the solution pH monitoring with pH-ISFET. In this way, we demonstrated the possibility to detect bacterial activity in Lactobacillus Acidophilus case and so, we began to determine bacterium biological hallmark. Second approach relies on ChemFET adaptation to enzymatic detection. In this way, we planed to use a polymer as physical support for biological material. So, classical photolithography techniques allow us to make enzymatic layers using PVA in order to realize biochemical detection. Following implementation of this process to urease, we characterized enzymatic activity evolution in the so fabricated membranes. Next, we validated this process fabricating ENFET chemical microsensors. Then, we were able to detect urea rates thanks to pH variations within solutions with various concentrations.
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https://tel.archives-ouvertes.fr/tel-00142498
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Submitted on : Thursday, April 19, 2007 - 2:26:12 PM
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  • HAL Id : tel-00142498, version 1

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Marie Laure Pourciel-Gouzy. Développement d'interfaces adaptées aux analyses biochimiques et biologiques. Application aux capteurs chimiques CHEMFETs. Micro et nanotechnologies/Microélectronique. INSA de Toulouse, 2004. Français. ⟨tel-00142498⟩

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