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Quelques expériences entre physique, chimie, biologie :
formations de boucles dans l'ADN, moteurs moléculaires et
ségrégation chromosomique, excitation biphotonique,
spectroscopie de corrélation de fluorescence...

Abstract : In the first part, we describe the use of the single DNA micromanipulation
technique called magnetic tweezers to study the loop formation
kinetics and thermodynamics in DNA caused by the GalR repressor.We also
studied the properties of the DNA translocase FtsK involved in chromosome
segregation in E. coli. In a second part we present experiments using two
photons excitation. In a first set of experiments we did built an apparatus to
perform two photons cross section absorption for molecules synthesized in
the laboratory. Then we used fluorescence correlation spectroscopy to measure
diffusion coefficents and kinetics constants.
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https://tel.archives-ouvertes.fr/tel-00141893
Contributor : Jean Francois Allemand <>
Submitted on : Monday, April 16, 2007 - 4:08:46 PM
Last modification on : Thursday, December 10, 2020 - 12:37:50 PM
Long-term archiving on: : Tuesday, April 6, 2010 - 11:23:42 PM

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  • HAL Id : tel-00141893, version 1

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Jean Francois Allemand. Quelques expériences entre physique, chimie, biologie :
formations de boucles dans l'ADN, moteurs moléculaires et
ségrégation chromosomique, excitation biphotonique,
spectroscopie de corrélation de fluorescence.... Biophysique [physics.bio-ph]. Université Pierre et Marie Curie - Paris VI, 2006. ⟨tel-00141893⟩

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