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La teneur en lipides du régime affecte les capacités
d'absorption intestinale et la triglycéridémie postprandiale: contribution du récepteur nucléaire PPARβ ?

Abstract : The dietary long chain fatty acid have crucial functions into the organism such as energy
source, eicosanoïdes synthesis, gene regulation). The fat disposal is essential and depend on
intestinal barrier. It is well known that intestinal fat absorption is efficient. However, we don't
if the high triglycerides bioavailability of gut is attributable to inborn properties or to acquired
properties. To answer this question, mice were subjected to a high-fat diet (40%, w/w) during
21 days. We have shown that high-fat induces : 1) intestinal LCFA uptake, 2) intestinal
mitotic index which leads to an increase of intestinal relative mass, 3) expression of genes
involved in fatty uptake (Fatty Acid Transport Protein 4, FATP-4), trafficking (Fatty Acid
Transporter, FAT; Intestinal and Liver Fatty Acid-Binding Protein, I et L-FABP) and
lipoprotein synthesis (Microsomal Triglyceride transfer Protein, MTP et apolipoprotein AIV).
These changes were lipid-mediated, in that they were fully abolished in mice refed the
control diet. Moreover, these changes induces a higher efficiency of triglycerides clearance in
blood. The molecular mechanism at the origin of this intestinal adaptation could be the
nuclear receptor, Peroxisome Proliferator-Activated Receptor β (PPARβ). To explore this
hypothesis, we have generated by the Cre-lox strategy a transgenic mouse model that
overexpressed specifically PPARβ in the small intestine. We have shown that the
overexpression of PPARβ led to a low fat-mediated adaptation. According to our hypothesis,
this data might result from an alteration of cell differentiation.
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https://tel.archives-ouvertes.fr/tel-00141891
Contributor : Sabine Gros <>
Submitted on : Monday, April 16, 2007 - 4:06:27 PM
Last modification on : Friday, February 9, 2018 - 1:56:03 PM
Long-term archiving on: : Friday, November 25, 2016 - 3:55:12 PM

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  • HAL Id : tel-00141891, version 1

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Valérie Petit. La teneur en lipides du régime affecte les capacités
d'absorption intestinale et la triglycéridémie postprandiale: contribution du récepteur nucléaire PPARβ ?. domain_other. Université de Bourgogne, 2007. Français. ⟨tel-00141891⟩

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