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Suivi de molécules uniques à l'aide de nanocristaux semiconducteurs :
méthodes et application à l'étude de la dynamique du récepteur de la glycine

Abstract : Single molecule tracking experiments are useful to understand biological processes since they
give access to dynamic properties which are inaccessible with ensemble measurements. We present
the properties of semiconductor quantum dots (QDs) and show how their superior brightness and
photostability enable ultrasensitive biological imaging.
Next, we introduce a new approach for automatically tracking QD-labeled membrane molecules
which accounts for the blinking of the fluorescent probes and we discuss methods specifically adapted
to analyse the obtained trajectories.
Finally, we apply these sensitive imaging techniques to study glycine receptors (GlyR) lateral
dynamics in live cells. We discuss a study on the regulation of GlyR number at synapses by the cytoskeleton.
We also investigate the interactions between GlyR and gephyrin (its scaffolding protein)
in model cellular systems and we characterize the corresponding equilibrium.
Complete list of metadatas

https://tel.archives-ouvertes.fr/tel-00141586
Contributor : Marie-Virginie Ehrensperger <>
Submitted on : Friday, April 13, 2007 - 12:44:36 PM
Last modification on : Thursday, December 10, 2020 - 12:36:46 PM
Long-term archiving on: : Tuesday, April 6, 2010 - 10:34:07 PM

Identifiers

  • HAL Id : tel-00141586, version 1

Citation

Marie-Virginie Ehrensperger. Suivi de molécules uniques à l'aide de nanocristaux semiconducteurs :
méthodes et application à l'étude de la dynamique du récepteur de la glycine. Biophysique [physics.bio-ph]. Université Pierre et Marie Curie - Paris VI, 2007. Français. ⟨tel-00141586⟩

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