EXPRESSION, PURIFICATION ET ÉTUDE STRUCTURALE PAR RMN DE PEBP DE DROSOPHILE

Abstract : Phosphatidylethanolamine Binding Proteins (PEBPs) are proteins of about 20 kDa highly conserved proteins in all three kingdoms from bacteria to mammals. They are characterized by their capacity to bind anionic ligands. In addition PEBPs seem to be involved in numerous cellular processes (mostly in signalling pathways), and physiological processes (such as spermatogenesis) of the healthy organism. However modifications of the level of PEBPs expression are correlated with pathological situations like primary and metastatic cancers, diabete, Alzheimer disease and infertility.
Seven PEBPs have been isolated in drosophila. During this PhD thesis three of them have been expressed in Escherichia coli and purification protocole have been developed to produce the protein in large quantity. One of them, CG7054, was selected for a structural study by NMR. The assignment of 'H, 13C and 'Shi resonances was performed for the backbone and side chair atome. The backbone dynamics was studied and the 3D structure was determined and compared to other PEBPs.
Document type :
Theses
Complete list of metadatas

Cited literature [132 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-00139424
Contributor : Patricia Leloup <>
Submitted on : Friday, March 30, 2007 - 4:15:39 PM
Last modification on : Friday, November 16, 2018 - 9:22:01 PM
Long-term archiving on : Wednesday, April 7, 2010 - 1:48:49 AM

Identifiers

  • HAL Id : tel-00139424, version 1

Citation

Rautureau Gilles. EXPRESSION, PURIFICATION ET ÉTUDE STRUCTURALE PAR RMN DE PEBP DE DROSOPHILE. Autre [q-bio.OT]. Université d'Orléans, 2006. Français. ⟨tel-00139424⟩

Share

Metrics

Record views

327

Files downloads

1063