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Effet d'un traitement à la morphine sur le protéome des cellules de neuroblastome humain SH-SY5Y

Abstract : Morphine is widely used for its pharmacologic properties but a chronic treatment leads to side effects such as tolerance and dependence which restrict clinical use. In order to understand the molecular basis of these side effects, we have analysed the impacts of a morphine treatment on the proteome and phosphoproteome prepared from SH-SY5Y cells. Using a differential proteomic strategy we have shown that chronic drug treatment induces a proteasomal dependent degradation of Gbeta and Ggamma2 proteins. Furthermore, G beta degradation was highly correlated with adenylyl cyclase sensitization, a phenomenon known to be involved in morphine dependence. Overall, our proteomic analysis has detected about fifty proteins and hundred phosphoproteins modulated by a morphine treatment. In conclusion, my PhD work has highlighted a new molecular mechanism underlying adenylyl cyclase sensitization and suggests for the first time that the proteasome pathway is involved in long-term morphine adaptations.
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https://tel.archives-ouvertes.fr/tel-00137683
Contributor : Jérémie Neasta <>
Submitted on : Wednesday, March 21, 2007 - 11:48:20 AM
Last modification on : Thursday, October 15, 2020 - 9:39:35 AM
Long-term archiving on: : Friday, September 21, 2012 - 1:05:50 PM

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  • HAL Id : tel-00137683, version 1

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Jérémie Neasta. Effet d'un traitement à la morphine sur le protéome des cellules de neuroblastome humain SH-SY5Y. Biochimie [q-bio.BM]. Université Paul Sabatier - Toulouse III, 2006. Français. ⟨tel-00137683⟩

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