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Les lésions des acides nucléiques: détection par CLHP-SM/SM dans les milieux biologiques humains et intérêt comme biomarqueurs du stress oxydant et de l'inflammation.

Carine Badouard 1
1 LAN - Laboratoire Lésions des Acides Nucléiques
SCIB - UMR E3 - Service de Chimie Inorganique et Biologique : DSM/INAC/SCIB
Abstract : Molecules involved in oxidative stress or inflammation can damage DNA or RNA, the biopolymers that contain the genetic information. The cell has developed several enzymatic systems to repair the damage but some of them can remain and lead to mutagenecity. Hence, we focused our attention on the simultaneous quantification of several lesions using high performance liquid chromatography coupled to tandem mass spectrometry, a very sensitive, fast and reliable analytical system. The aim of this work was to develop new biomarkers of oxidative stress among the known DNA lesions by quantifying them in human biological fluids. For such a purpose, three types of DNA lesions were monitored: lesions due to oxidative stress, chlorinated lesions arising from inflammation processes and lesions generated subsequently to lipid peroxidation. The analytical method has been optimised and the biological validation was performed using different pathologies such as diabetes, patients suffered from cancer and treated by radiotherapy and men with infertility. Preliminary results obtained show different levels of DNA lesions between patients and healthy controls. These results have to be confirmed in order to confirm the significance of these lesions as potential biomarkers. Other kinds of pathologies will be investigated using the same approach.
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https://tel.archives-ouvertes.fr/tel-00134563
Contributor : Carine Badouard <>
Submitted on : Friday, March 2, 2007 - 3:16:35 PM
Last modification on : Tuesday, May 11, 2021 - 11:36:06 AM

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  • HAL Id : tel-00134563, version 1

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Carine Badouard. Les lésions des acides nucléiques: détection par CLHP-SM/SM dans les milieux biologiques humains et intérêt comme biomarqueurs du stress oxydant et de l'inflammation.. Biochimie [q-bio.BM]. Université Joseph-Fourier - Grenoble I, 2006. Français. ⟨tel-00134563⟩

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