Skip to Main content Skip to Navigation
Theses

Imagerie spectroscopique et spectroscopie localisée par RMN du proton in vivo.
Application à l'étude de la physiopathologie tumorale par cartographie des métabolites et du pH extracellulaire dans un modèle experimental de gliomes C6

Abstract : The aim of this work was to optimize the conditions and the acquisition techniques for spectroscopy and spectroscopic imaging by 1H NMR in vivo in small animals and to apply these techniques to two biological topics. Acquisition conditions have been improved by the use of an automatic correction of B0 field inhomogeneities. Sequences have been improved by the use of an optimized water suppression scheme (VAPOR) and of spiral encoding gradients for short echo time spectroscopic imaging.
Extracellular pH (pHe) in tumors is more acidic than in normal tissue.This acidity contributes to tumor proliferation. With a new probe molecule, ISUCA, we used spectroscopic imaging to map the distribution of pHe in C6 gliomas and compared it with the distribution of lactate. The two distributions are not spatially correlated. After hyperglycemia, lactate and protons quantities are globally increased but with a negative spatial correlation. This result suggests that proton are redistributed from sites of glycolysis to membrane pumps.
The aim of the second project was to study the role of ammonium in the regulation of glycolysis in the brain. Hyperammonemia increases lactate concentration in the brain. I showed that this increase can be transient, which is a required condition for ammonium to play a physiological role in metabolic interaction between astrocytes and neurones.
Complete list of metadata

Cited literature [102 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-00134012
Contributor : Peggy Provent <>
Submitted on : Wednesday, February 28, 2007 - 2:35:19 PM
Last modification on : Friday, November 6, 2020 - 4:12:58 AM
Long-term archiving on: : Tuesday, April 6, 2010 - 9:39:02 PM

Identifiers

  • HAL Id : tel-00134012, version 1

Collections

INSERM | UJF | UGA

Citation

Peggy Provent. Imagerie spectroscopique et spectroscopie localisée par RMN du proton in vivo.
Application à l'étude de la physiopathologie tumorale par cartographie des métabolites et du pH extracellulaire dans un modèle experimental de gliomes C6. Biophysique [physics.bio-ph]. Université Joseph-Fourier - Grenoble I, 2006. Français. ⟨tel-00134012⟩

Share

Metrics

Record views

427

Files downloads

1209