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Structure et fonction des hélicases de la famille RecQ : rôle du doigt de zinc dans la régulation des activités enzymatiques

Abstract : The RecQ family of the DNA helicases is involved in the maintenance of genomic stability. During this work, we analyzed the function of the zinc finger of the RecQ Ct domain of the RecQ family helicases. We showed that the zinc finger is involved in DNA binding and in the correct folding of the RecQ protein. It has been shown for the first time that the RECQ5 helicase domain has an intrinsically high ssDNA annealing activity and the zinc finger motif regulates the DNA binding, ATPase, unwinding, strand annealing and strand exchange activities of the helicase domain. The presence of the zinc finger is essential for the ATPase and helicase activity, but not for the DNA annealing activity. Our works on the RecQ helicases from Bacillus subtilis also support this conclusion.
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https://tel.archives-ouvertes.fr/tel-00133625
Contributor : Ens Cachan Bibliothèque <>
Submitted on : Tuesday, February 27, 2007 - 10:33:59 AM
Last modification on : Thursday, July 2, 2020 - 5:40:10 PM
Long-term archiving on: : Wednesday, April 7, 2010 - 12:47:03 AM

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  • HAL Id : tel-00133625, version 1

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Jie Lin Liu. Structure et fonction des hélicases de la famille RecQ : rôle du doigt de zinc dans la régulation des activités enzymatiques. Biochimie [q-bio.BM]. École normale supérieure de Cachan - ENS Cachan, 2006. Français. ⟨tel-00133625⟩

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