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Régulation de l'activité de la protéine Rev du HIV-1 par des modifications post-traductionnelles et inhibition de sa fonction par des peptides actifs à partir du milieu extracellulaire

Abstract : The HIV-1 Rev protein plays a key role in virus replication by allowing export to the cytoplasm of unspliced or singly-spliced mRNAs. To inhibit its function, we developed peptides which are able to recognize Rev and to inhibit its function, named SHPR (Sumo Heptapeptide Protein transduction domain for binding Rev). We showed that these peptides are able to passively penetrate within cells and to reach the nucleus, where they induce Rev degradation, mostly by the proteasome pathway. Through mutagenesis experiments, we determined the contribution of each of the SHPR domains to the antiviral effect, and demonstrated that few of them can be optimized so as to enhance SHPR efficiency. We have also shown that, whereas Rev is not regulated by sumoylation, it can be modified by addition of a polyubiquitin chain, which stabilizes the protein but hinders
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https://tel.archives-ouvertes.fr/tel-00130625
Contributor : Anne-Laure Vitte <>
Submitted on : Tuesday, February 13, 2007 - 9:44:26 AM
Last modification on : Sunday, June 7, 2020 - 4:15:53 AM
Long-term archiving on: : Tuesday, April 6, 2010 - 11:50:44 PM

Identifiers

  • HAL Id : tel-00130625, version 1
  • PRODINRA : 251932

Citation

Anne-Laure Vitte. Régulation de l'activité de la protéine Rev du HIV-1 par des modifications post-traductionnelles et inhibition de sa fonction par des peptides actifs à partir du milieu extracellulaire. Biologie cellulaire. Ecole normale supérieure de lyon - ENS LYON, 2006. Français. ⟨tel-00130625⟩

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