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Immobilisation d'enzymes dans des films de polymère conducteur : le PEDT. Application à la réalisation de biocapteurs ampérométriques pour le dosage du glucose et de composés phénoliques

Abstract : The amperometric biosensors presented in this work are based on immobilization of oxido-reductase enzymes in a conducting polymer deposited on electrode surface. The biosensor performance depends on the immobilization method and the characteristics of the resulting matrix. An electrochemical polymerization technique was used to entrap the enzymes in poly(3,4 ethylenedioxythiophene) (PEDT) a polythiophene derivative. Potentialities of this technique were demonstrated with two enzymes : glucose oxidase and polyphenol oxidase. Optimized conditions were obtained first with a biosensor operating in batch mode and then in flow injection analysis (FIA). This latter is quite suitable for monitoring of glucose in biological samples. Selectivity of this biosensor in the presence of interferent species was improved by enhancing the response to glucose with electrode platinization or the use of redox mediators. Enzymatic amplification involved in polyphenol oxidase system allowed the corresponding biosensor to detect phenolic pollutants down to 50nM.
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Submitted on : Monday, January 29, 2007 - 10:54:14 AM
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  • HAL Id : tel-00127122, version 1

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Silvia Fabiano. Immobilisation d'enzymes dans des films de polymère conducteur : le PEDT. Application à la réalisation de biocapteurs ampérométriques pour le dosage du glucose et de composés phénoliques. Autre. Ecole Nationale Supérieure des Mines de Saint-Etienne, 2002. Français. ⟨tel-00127122⟩

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