Skip to Main content Skip to Navigation
Theses

Synthèse chimio-enzymatique de dérivés de laminari-oligosaccharides,
et leur utilisation biochimique

Abstract : ?-(1,3)-glucans are found in a wide range of plants and have been recognised for their immunostimulating activities in mammals, as well as for their role in plant defence system. The first chapter of this manuscript is a short review on glycoside-hydrolases and, particularly on glycosynthases. Glucan-binding proteins, enzymes implied in ?-glucan recognition, are also introduced, as well as the knowledge acquired on ?-(1,3)-glucans and ?-(1,3)-gluco-oligosaccharides. The second chapter describes the work done in the CERMAV (Grenoble), in order to obtain the necessary tools to study ?-(1,3)-glucans and ?-(1,3)-gluco-oligosaccharides action modes. The synthesis of linear ?-(1,3)-gluco-oligosaccharides, of controled DP, using the ?-(1,3)-glucosynthase GII E231G, and the synthesis of a fluorogenic substrate for endo-?-(1,3)-glucanase activity study, are described. The third chapter is about ?-glucan-binding proteins, and describes the work done in the botanical institute of the LMU (Munich), for the european research project SACC-SIG-NET (n°HPRN-CT-2202-00251). This research deals, on one hand, with the study of the Glycine max ?-glucan-binding protein, which allowed to determined for the first time the hydrolysis molecular mechanism of a glycoside-hydrolase belonging to the GH-81 family. On the other hand, a cloning work on the Gbps gene family of Medicago truncatula, a model plant for the Fabaceae, is presented.
Document type :
Theses
Complete list of metadatas

Cited literature [149 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-00123485
Contributor : Emilie Montel <>
Submitted on : Tuesday, January 9, 2007 - 5:47:51 PM
Last modification on : Friday, November 6, 2020 - 3:52:35 AM
Long-term archiving on: : Tuesday, April 6, 2010 - 7:43:07 PM

Identifiers

  • HAL Id : tel-00123485, version 1

Collections

Citation

Emilie Montel. Synthèse chimio-enzymatique de dérivés de laminari-oligosaccharides,
et leur utilisation biochimique. Autre. Université Joseph-Fourier - Grenoble I, 2006. Français. ⟨tel-00123485⟩

Share

Metrics

Record views

426

Files downloads

1841