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Microscopie par diffusion cohérente Raman
CARS: Application à l'imagerie des milieux
biologiques

Abstract : Coherent anti-Stokes Raman scattering microscopy (CARS) is a new ap-
proach for chemical imaging of molecular systems, with high sensitivity, high
spatial resolution, and three dimensional sectioning capability, without using
fluorophores that are prone to photobleaching. This technique permits to map
selectively molecular species, by using vibrational properties of their chemical
bounds. CARS is described by a four wave-mixing process, where the signal
intensity depends nonlinearly on the incident intensities, and generated in a
direction determined by the phase-matching condition. The approach of this
work was to realize a CARS microscope, allowing biological systems ima-
ging without any labelling or staining. Studies were undertaken showing the
potentialities of this tool, as well as its characterization in the spatial and
spectral domain.
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https://tel.archives-ouvertes.fr/tel-00121943
Contributor : Nadia Djaker <>
Submitted on : Friday, December 22, 2006 - 4:07:24 PM
Last modification on : Thursday, January 23, 2020 - 6:22:07 PM
Long-term archiving on: : Tuesday, April 6, 2010 - 9:29:57 PM

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  • HAL Id : tel-00121943, version 1

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Nadia Djaker. Microscopie par diffusion cohérente Raman
CARS: Application à l'imagerie des milieux
biologiques. Biophysique [physics.bio-ph]. Université de droit, d'économie et des sciences - Aix-Marseille III, 2006. Français. ⟨tel-00121943⟩

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