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Analyse locale des sensibilités des lectures angulaires, spectroscopiques et ellipsométriques de la Résonance des Plasmons de Surface en vue de la miniaturisation des biocapteurs optiques

Abstract : The fluorescence labelling, used by the fluorescence biochips, is known to change the charge distribution of the labelled molecules and then modify their biological activity. Among label free biosensors, we choose an optical detection, such as spectroscopic ellipsometry and/or surface plasmon resonance (SPR) of the biolayers.
These optical techniques are based on the measurement of both thickness and optical index of the adsorbed biolayer. An AFM statistical measurement of the thickness of functionalized lithographed microstructures has been performed in order to determine the thickness of the biolayers.
We have shown that the spectroscopic reading of the SPR phase shift is 100 times more sensitive than a classical angular reading and allows to determine both the thickness and the optical index of the biolayers. The miniaturization of these sensors has been achieved following two RPS microscopy techniques :
- CCD far field imaging
- Spectroscopic Ellipsometric Scanning microscopy
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https://tel.archives-ouvertes.fr/tel-00115725
Contributor : Guillaume Legay <>
Submitted on : Wednesday, November 22, 2006 - 5:49:20 PM
Last modification on : Wednesday, July 25, 2018 - 1:22:59 AM
Long-term archiving on: : Tuesday, April 6, 2010 - 11:16:08 PM

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Guillaume Legay. Analyse locale des sensibilités des lectures angulaires, spectroscopiques et ellipsométriques de la Résonance des Plasmons de Surface en vue de la miniaturisation des biocapteurs optiques. Biophysique [physics.bio-ph]. Université de Bourgogne, 2006. Français. ⟨tel-00115725⟩

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