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Réparation de modèles de lésions photoinduites de l'ADN. Approches électrochimiques.

Abstract : The goal of this work is to better understand the repair mechanism of photoinduced lesions in DNA (cyclobutane dimers and pyrimidine (6-4) pyrimidone adducts) by photolyase redox enzymes, using tools and concepts of molecular electrochemistry.
Thanks to the study of model compounds of cyclobutane lesions by cyclic voltametry, we have been able to mimic the key step of the enzymatic repair (dissociative electron transfer) and to monitor the repair of model compounds by Escherichia coli DNA photolyase. From these results, we have discussed the repair mechanism, especially the stepwise or concerted character of the process.
Repair mechanism of (6-4) adducts is not known at time, but a possible pathway implies an electron transfer coupled to the cleavage of two bonds in the closed form of the lesions (oxetanes). Voltametric study of reduction and oxidation of model oxetanes and their repair by E. coli DNA photolyase gave some experimental evidence confirming the proposed mechnism and allowing a better understanding of it.
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https://tel.archives-ouvertes.fr/tel-00109091
Contributor : Fabien Boussicault <>
Submitted on : Monday, October 23, 2006 - 5:37:56 PM
Last modification on : Wednesday, October 21, 2020 - 9:40:04 AM
Long-term archiving on: : Tuesday, April 6, 2010 - 8:43:31 PM

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  • HAL Id : tel-00109091, version 1

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Fabien Boussicault. Réparation de modèles de lésions photoinduites de l'ADN. Approches électrochimiques.. Autre. Université Paris-Diderot - Paris VII, 2006. Français. ⟨tel-00109091⟩

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