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BASES MOLECULAIRES DE LA VARIEGATION D'EFFET
DE POSITION CHEZ DROSOPHILA MELANOGASTER

Abstract : Satellite III sequences (SATIII) represent the bulk of drosophila X chromosome heterochromatin
and play a key role in position effect variegation (PEV) of the white gene in the white-mottled
strain. Their structural characteristics, based on their biased dA•dT rich nucleotidic composition,
make them good targets for artificial DNA minor groove binders. Such new tools allowed us to
improve the SATIII sequences' function in heterochromatic structure formation. Our work allows
us to shed light on the functional role of the D1 protein, which specifically binds SAT III
sequences, as well as topoisomerase II, in the initiation of the heterochromatin assembly. Our
results suggest that SATIII sequences represent a reservoir for proteins regulating ribosomal
DNA arrays (rDNA) transcription located next to SATIII sequences. We also show involvement
of a boundary element in heterochromatin partitioning, which plays a role in regulation of rDNA
expression.
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https://tel.archives-ouvertes.fr/tel-00107128
Contributor : Chantal Michel <>
Submitted on : Tuesday, October 17, 2006 - 2:45:48 PM
Last modification on : Wednesday, January 20, 2021 - 3:26:04 PM
Long-term archiving on: : Tuesday, April 6, 2010 - 6:15:10 PM

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  • HAL Id : tel-00107128, version 1

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Roxane Blattes. BASES MOLECULAIRES DE LA VARIEGATION D'EFFET
DE POSITION CHEZ DROSOPHILA MELANOGASTER. Biochimie [q-bio.BM]. Université Paul Sabatier - Toulouse III, 2006. Français. ⟨tel-00107128⟩

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