Skip to Main content Skip to Navigation
Theses

Inférence fonctionnelle et prédiction de voies métaboliques.
Application à la bactérie fixatrice d'azote
Sinorhizobium meliloti.

Abstract : Complete genomes of bacteria are sequenced in growing number. In parallel programs of systematic analysis of genes and proteins expression in different conditions are installed.
Understanding of organism functioning requires the annotation of genes functions and the integration of this data in functional diagram. Metabolic pathways constitute a class of functions allowing tackling this integration problem, these are well listed in numerous organisms and are accessible to experimentation.
In a first time, we have developed an automated method for enzyme function detection. This method named PRIAM (PRofils pour l'Identification Automatique du Métabolisme) based on the classification of enzymes in the ENZYME database and relies on sets of position-specific scoring matrices (“profiles”) automatically tailored for each ENZYME entry. Then, this method allows to identify enzymes in a complete genome and to visualize the results on KEGG graphs.
In a second time, this method has been applied on the complete genome of the nitrogen fixing bacterium Sinorhizobium meliloti and allows facilitating the interpretation of specific metabolic pathways of this symbiotic organism.
Complete list of metadata

Cited literature [38 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-00104955
Contributor : Clotilde Claudel <>
Submitted on : Monday, October 9, 2006 - 6:26:40 PM
Last modification on : Friday, March 19, 2021 - 3:35:06 AM
Long-term archiving on: : Tuesday, April 6, 2010 - 7:05:56 PM

Identifiers

  • HAL Id : tel-00104955, version 1
  • PRODINRA : 318674

Collections

Citation

Clotilde Claudel. Inférence fonctionnelle et prédiction de voies métaboliques.
Application à la bactérie fixatrice d'azote
Sinorhizobium meliloti.. Biochimie [q-bio.BM]. Université Paul Sabatier - Toulouse III, 2003. Français. ⟨tel-00104955⟩

Share

Metrics

Record views

323

Files downloads

1921