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Réorganisation de l'épigénome associé à la spermiogénèse

Abstract : Each spermatozoon not only transmits the paternal genome, but also epigenetic information, closely related to the genome structural organization, or “epigenome”. Despite its crucial involvement in embryonic development, epigenome reprogramming during germ cells post-meiotic differentiation, or spermiogenesis, is a very poorly known process. It involves a global chromatin restructuration, characterized by extraction of most histones and their replacement by sperm-specific proteins.
Based on the study of post meiotic cells, this work shows an original dialogue between post-translational histone modifications, and the presence of new histone variants associated with pericentric heterochromatin. Epigenetic reprogramming of imprinting control regions is also analyzed. Moreover, new functions have been attributed to several chaperones, mainly HSP70.2, Npm3 and NAP1L4, which are involved in chromatin incorporation of histone variants and basic proteins during the late steps of spermiogenesis.
Altogether, a coordinated action of several chromatin reorganization pathways leads to the setting of a new epigenome, which will be transmitted to the zygote.
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https://tel.archives-ouvertes.fr/tel-00104147
Contributor : Jerome Govin <>
Submitted on : Thursday, October 5, 2006 - 6:27:01 PM
Last modification on : Friday, November 6, 2020 - 4:01:07 AM
Long-term archiving on: : Tuesday, April 6, 2010 - 6:36:34 PM

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  • HAL Id : tel-00104147, version 1

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Citation

Jerome Govin. Réorganisation de l'épigénome associé à la spermiogénèse. Biochimie [q-bio.BM]. Université Joseph-Fourier - Grenoble I, 2006. Français. ⟨tel-00104147⟩

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