.. Dynamique-des-protéines-au-cours-de-la-biogenèse-des-ribosomes-eucaryotes, 51 3.1. Composition des particules pré-ribosomiques, ., p.60

S. Ordre-d-'assemblage-des-facteurs-pré-60s:-l-'approche, 71 3.1. Limites techniques de l'analyse de l'ordre d'assemblage des facteurs pré-60S par chromatographie d'affinité en tandem, p.71

U. Réseau-fonctionnel-impliqué-dans-le-recyclage-des-facteurs-pré-60s-nucléocytoplasmiques and .. , 77 4.1. Étude de l'assemblage de la protéine ribosomique tardive Rpl24 sur la grande sous-unité ribosomique, p.77

F. Nsa2 and C. Instable, ARN 27SB ? Nsa2, an unstable, conserved factor required for the maturation of 27SB pre-rRNAs, p.137

.. Plasmides-utilisés-au-cours-de-cette-Étude, 175 5.1. Vecteurs d'entrée pour le système Vecteurs d'expression modifiés pour le système, p.179

C. Allmang, E. Petfalski, A. Podtelejnikov, M. Mann, D. Tollervey et al., The yeast exosome and human PM-Scl are related complexes of 3' right-arrow 5' exonucleases, Genes & Development, vol.13, issue.16, pp.2148-58, 1999.
DOI : 10.1101/gad.13.16.2148

J. S. Andersen, C. E. Lyon, A. H. Fox, A. K. Leung, Y. W. Lam et al., Nuclear export of 60s ribosomal subunits depends on Xpo1p and requires a nuclear export sequence-containing factor, Mol Cell Biol, vol.21, pp.3405-3420, 2001.

K. Galani, T. A. Nissan, E. Petfalski, D. Tollervey, and E. E. Hurt, Rea1, a Dynein-related Nuclear AAA-ATPase, Is Involved in Late rRNA Processing and Nuclear Export of 60 S Subunits, Journal of Biological Chemistry, vol.279, issue.53, pp.55411-55419, 2004.
DOI : 10.1074/jbc.M406876200

A. C. Gavin, M. Bosche, R. Krause, P. Grandi, M. Marzioch et al., Functional organization of the yeast proteome by systematic analysis of protein complexes, Nature, vol.415, issue.6868, pp.141-148, 2002.
DOI : 10.1038/415141a

T. H. Geerlings, J. C. Vos, and H. A. Raue, The final step in the formation of 25S rRNA in Saccharomyces cerevisiae is performed by 5??? ??? 3??? exonucleases, RNA, vol.6, issue.12, pp.1698-703, 2000.
DOI : 10.1017/S1355838200001540

D. Gelperin, L. Horton, J. Beckman, J. Hensold, and S. K. Lemmon, Bms1p, a novel GTP-binding protein, and the related Tsr1p are required for distinct steps of 40S ribosome biogenesis in yeast, RNA, vol.7, issue.9, pp.1268-1283, 2001.
DOI : 10.1017/S1355838201013073

S. Ghaemmaghami, W. K. Huh, K. Bower, R. W. Howson, A. Belle et al., Global analysis of protein expression in yeast, Nature, vol.425, issue.6959, pp.737-778, 2003.
DOI : 10.1038/nature02046

R. L. Gourse, H. A. De-boer, and E. M. Nomura, DNA determinants of rRNA synthesis in E. coli: Growth rate dependent regulation, feedback inhibition, upstream activation, antitermination, Cell, vol.44, issue.1, pp.197-205, 1986.
DOI : 10.1016/0092-8674(86)90498-8

P. Grandi, V. Rybin, J. Bassler, E. Petfalski, D. Strauss et al., 90S Pre-Ribosomes Include the 35S Pre-rRNA, the U3 snoRNP, and 40S Subunit Processing Factors but Predominantly Lack 60S Synthesis Factors, Molecular Cell, vol.10, issue.1, pp.105-120, 2002.
DOI : 10.1016/S1097-2765(02)00579-8

S. Granneman, K. A. Bernstein, F. Bleichert, and S. J. Baserga, Comprehensive Mutational Analysis of Yeast DEXD/H Box RNA Helicases Required for Small Ribosomal Subunit Synthesis, Molecular and Cellular Biology, vol.26, issue.4, pp.1183-94, 2006.
DOI : 10.1128/MCB.26.4.1183-1194.2006

F. Gros, H. Hiatt, and E. W. Gilbert, Unstable Ribonucleic Acid Revealed by Pulse Labelling of Escherichia Coli, Nature, vol.46, issue.4776, pp.581-585, 1961.
DOI : 10.1016/0042-6822(58)90101-6

B. Meagher, Y. Imai, C. J. Guo, J. Brame, D. F. Shabanowitz et al., Composition and functional characterization of yeast 66S ribosome assembly intermediates, Release of the export adapter, Nmd3p, from the 60S ribosomal subunit requires Rpl10p and the cytoplasmic GTPase Lsg1p, pp.505-520, 2001.

W. A. Held, M. Nomura, and J. W. Hershey, Ribosomal protein S21 is required for full activity in the initiation of protein synthesis, MGG Molecular & General Genetics, vol.111, issue.1, pp.567-7911, 1974.
DOI : 10.1007/BF00267291

K. Karbstein, J. A. Et, D. A. Doudna-konings, and R. R. Et, GTP-dependent Formation of a Ribonucleoprotein Subcomplex Required for Ribosome Biogenesis A comparison of thermodynamic foldings with comparatively derived structures of 16S and 16S-like rRNAs, J Mol Biol. Rna, vol.83, issue.1, pp.559-74, 1995.

M. Kozak, How do eucaryotic ribosomes select initiation regions in messenger RNA? Cell, pp.1109-1132, 1978.

M. Kozak, Evaluation of the ???scanning model??? for initiation of protein synthesis in eucaryotes, Cell, vol.22, issue.1, pp.7-8, 1980.
DOI : 10.1016/0092-8674(80)90148-8

D. Kressler, M. Rojo, P. Linder, and E. J. Cruz, Spb1p is a putative methyltransferase required for 60S ribosomal subunit biogenesis in Saccharomyces cerevisiae, Nucleic Acids Research, vol.27, issue.23, pp.4598-608, 1999.
DOI : 10.1093/nar/27.23.4598

V. Zhang, D. P. Canadien, B. K. Richards, A. Beattie, W. Lalev et al., High-definition macromolecular composition of yeast RNA-processing complexes, Mol Cell, vol.13, pp.225-264, 2004.

T. Kruiswijk, R. J. Planta, and J. M. Krop, The course of the assembly of ribosomal subunits in yeast, Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, vol.517, issue.2, pp.378-89, 1978.
DOI : 10.1016/0005-2787(78)90204-6

J. Kufel, C. Allmang, E. Petfalski, J. Beggs, and E. D. Tollervey, Lsm Proteins Are Required for Normal Processing and Stability of Ribosomal RNAs, Journal of Biological Chemistry, vol.278, issue.4, pp.2147-56, 2003.
DOI : 10.1074/jbc.M208856200

J. Kufel, B. Dichtl, and E. D. Tollervey, Yeast Rnt1p is required for cleavage of the pre-ribosomal RNA in the 3??? ETS but not the 5??? ETS, RNA, vol.5, issue.7, pp.909-926, 1999.
DOI : 10.1017/S135583829999026X

S. Labeit, B. Et, and . Kolmerer, Titins: Giant Proteins in Charge of Muscle Ultrastructure and Elasticity, Science, vol.270, issue.5234, pp.293-299, 1995.
DOI : 10.1126/science.270.5234.293

D. Lafontaine, J. Delcour, A. L. Glasser, J. Desgres, and E. J. Vandenhaute, The DIM1 Gene Responsible for the Conserved m62Am62A Dimethylation in the 3???-Terminal Loop of 18 S rRNA is Essential in Yeast, Journal of Molecular Biology, vol.241, issue.3, pp.492-499, 1994.
DOI : 10.1006/jmbi.1994.1525

D. Lafontaine, J. Vandenhaute, and E. D. Tollervey, The 18S rRNA dimethylase Dim1p is required for pre-ribosomal RNA processing in yeast., Genes & Development, vol.9, issue.20, pp.2470-81, 1995.
DOI : 10.1101/gad.9.20.2470

D. L. Lafontaine, T. Preiss, and E. D. Tollervey, Yeast 18S rRNA Dimethylase Dim1p: a Quality Control Mechanism in Ribosome Synthesis?, Molecular and Cellular Biology, vol.18, issue.4, pp.2360-70, 1998.
DOI : 10.1128/MCB.18.4.2360

D. L. Lafontaine, D. Et, and . Tollervey, The function and synthesis of ribosomes, Nature Reviews Molecular Cell Biology, vol.66, issue.7, pp.514-534, 2001.
DOI : 10.1038/35080045

B. Lapeyre, S. K. Et, and . Purushothaman, Spb1p-Directed Formation of Gm2922 in the Ribosome Catalytic Center Occurs at a Late Processing Stage, Molecular Cell, vol.16, issue.4, pp.663-672, 2004.
DOI : 10.1016/j.molcel.2004.10.022

N. Laronde-leblanc, A. Et, and . Wlodawer, A Family Portrait of the RIO Kinases, Journal of Biological Chemistry, vol.280, issue.45, pp.37297-300, 2005.
DOI : 10.1074/jbc.R500013200

A. Lebreton, C. Saveanu, L. Decourty, A. Jacquier, and M. Fromont-racine, Nsa2 Is an Unstable, Conserved Factor Required for the Maturation of 27 SB Pre-rRNAs, Journal of Biological Chemistry, vol.281, issue.37, pp.27099-108, 2006.
DOI : 10.1074/jbc.M602199200
URL : https://hal.archives-ouvertes.fr/hal-01350676

A. Lebreton, C. Saveanu, L. Decourty, J. C. Rain, A. Jacquier et al., A functional network involved in the recycling of nucleocytoplasmic pre-60S factors, The Journal of Cell Biology, vol.257, issue.3, pp.349-360, 2006.
DOI : 10.1016/S0962-8924(03)00054-0
URL : https://hal.archives-ouvertes.fr/hal-01350674

I. Leger-silvestre, S. Trumtel, J. Noaillac-depeyre, and E. N. Gas, Functional compartmentalization of the nucleus in the budding yeast Saccharomyces cerevisiae, Chromosoma, vol.108, issue.2, pp.103-116, 1999.
DOI : 10.1007/s004120050357

D. M. Leslie, B. Grill, M. P. Rout, R. W. Wozniak, and J. D. Aitchison, Kap121p-Mediated Nuclear Import Is Required for Mating and Cellular Differentiation in Yeast, Molecular and Cellular Biology, vol.22, issue.8, pp.2544-55, 2002.
DOI : 10.1128/MCB.22.8.2544-2555.2002

D. M. Leslie, W. Zhang, B. L. Timney, B. T. Chait, M. P. Rout et al., Characterization of Karyopherin Cargoes Reveals Unique Mechanisms of Kap121p-Mediated Nuclear Import, Molecular and Cellular Biology, vol.24, issue.19, pp.8487-503, 2004.
DOI : 10.1128/MCB.24.19.8487-8503.2004

S. C. Li, C. L. Squires, and C. Squires, Antitermination of E. coli rRNA transcription is caused by a control region segment containing lambda nut-like sequences, Cell, vol.38, issue.3, pp.851-60, 1984.
DOI : 10.1016/0092-8674(84)90280-0

X. Li and Y. H. Chang, Amino-terminal protein processing in Saccharomyces cerevisiae is an essential function that requires two distinct methionine aminopeptidases., Proceedings of the National Academy of Sciences, vol.92, issue.26, pp.12357-61, 1995.
DOI : 10.1073/pnas.92.26.12357

Z. Lygerou, C. Allmang, D. Tollervey, and E. B. Seraphin, Accurate Processing of a Eukaryotic Precursor Ribosomal RNA by Ribonuclease MRP in Vitro, Science, vol.272, issue.5259, pp.268-70, 1996.
DOI : 10.1126/science.272.5259.268

P. A. Marks, E. R. Burka, and E. D. Schlessinger, PROTEIN SYNTHESIS IN ERYTHROID CELLS, I. RETICULOCYTE RIBOSOMES ACTIVE IN STIMULATING AMINO ACID INCORPORATION, Proceedings of the National Academy of Sciences, vol.48, issue.12, pp.2163-2171, 1962.
DOI : 10.1073/pnas.48.12.2163

D. Masson, T. E. Et, and . Kreis, Identification and molecular characterization of E-MAP-115, a novel microtubule-associated protein predominantly expressed in epithelial cells, The Journal of Cell Biology, vol.123, issue.2, pp.357-71, 1993.
DOI : 10.1083/jcb.123.2.357

Y. Matsuo, T. Morimoto, M. Kuwano, P. C. Loh, T. Oshima et al., The GTP-binding Protein YlqF Participates in the Late Step of 50 S Ribosomal Subunit Assembly in Bacillus subtilis, Journal of Biological Chemistry, vol.281, issue.12, 2006.
DOI : 10.1074/jbc.M512556200

B. Mcclintock, The relation of a particular chromosomal element to the development of the nucleoli in Zea mays, Zeitschrift f???r Zellforschung und Mikroskopische Anatomie, vol.36, issue.2, pp.294-328, 1934.
DOI : 10.1007/BF00374060

P. Mitchell, E. Petfalski, A. Shevchenko, M. Mann, and E. D. Tollervey, The Exosome: A Conserved Eukaryotic RNA Processing Complex Containing Multiple 3??????5??? Exoribonucleases, Cell, vol.91, issue.4, pp.457-66, 1997.
DOI : 10.1016/S0092-8674(00)80432-8

P. Mitchell, E. Petfalski, and E. D. Tollervey, The 3' end of yeast 5.8S rRNA is generated by an exonuclease processing mechanism., Genes & Development, vol.10, issue.4, pp.502-515, 1996.
DOI : 10.1101/gad.10.4.502

K. Mizuta, J. R. Et, and . Warner, Continued functioning of the secretory pathway is essential for ribosome synthesis., Molecular and Cellular Biology, vol.14, issue.4, pp.2493-502, 1994.
DOI : 10.1128/MCB.14.4.2493

T. H. Montgomery, Comparative cytological studies, with especial regard to the morphology of the nucleolus, Journal of Morphology, vol.2, issue.2, pp.265-582, 1898.
DOI : 10.1002/jmor.1050150204

T. Morimoto, P. C. Loh, T. Hirai, K. Asai, K. Kobayashi et al., Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis b, Microbiology, vol.148, issue.11, pp.3539-52, 2002.
DOI : 10.1099/00221287-148-11-3539

K. H. Nierhaus, The assembly of prokaryotic ribosomes, Biochimie, vol.73, issue.6, pp.739-55, 1991.
DOI : 10.1016/0300-9084(91)90054-5

L. Nilsson, A. Vanet, E. Vijgenboom, and E. L. Bosch, The role of FIS in trans activation of stable RNA operons of E. coli, Embo J, vol.9, pp.727-761, 1990.

M. W. Nirenberg and J. H. Matthaei, The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides, Proceedings of the National Academy of Sciences, vol.47, issue.10, pp.1588-602, 1961.
DOI : 10.1073/pnas.47.10.1588

T. A. Nissan, J. Bassler, E. Petfalski, D. Tollervey, and E. E. Hurt, 60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasm, The EMBO Journal, vol.21, issue.20, pp.5539-5547, 2002.
DOI : 10.1093/emboj/cdf547

H. F. Noller, Structure of Ribosomal RNA, Annual Review of Biochemistry, vol.53, issue.1, pp.119-62, 1984.
DOI : 10.1146/annurev.bi.53.070184.001003

M. Nomura, Regulation of ribosome biosynthesis in Escherichia coli and Saccharomyces cerevisiae: diversity and common principles, J Bacteriol, vol.181, pp.6857-64, 1999.

M. Oeffinger, M. Dlakic, and E. D. Tollervey, A pre-ribosome-associated HEAT-repeat protein is required for export of both ribosomal subunits, Genes & Development, vol.18, issue.2, pp.196-209, 2004.
DOI : 10.1101/gad.285604

M. O. Olson, The Nucleolus. Kluwer Academic / Plenum, 2004.

S. E. Ong, B. Blagoev, I. Kratchmarova, D. B. Kristensen, H. Steen et al., Stable Isotope Labeling by Amino Acids in Cell Culture, SILAC, as a Simple and Accurate Approach to Expression Proteomics, Molecular & Cellular Proteomics, vol.1, issue.5, pp.376-86, 2002.
DOI : 10.1074/mcp.M200025-MCP200

G. E. Palade, A SMALL PARTICULATE COMPONENT OF THE CYTOPLASM, The Journal of Cell Biology, vol.1, issue.1, pp.59-68, 1955.
DOI : 10.1083/jcb.1.1.59

F. Peske, A. Savelsbergh, V. I. Katunin, M. V. Rodnina, and E. W. Wintermeyer, Conformational Changes of the Small Ribosomal Subunit During Elongation Factor G-dependent tRNA???mRNA Translocation, Journal of Molecular Biology, vol.343, issue.5, pp.1183-94, 2004.
DOI : 10.1016/j.jmb.2004.08.097

R. D. Phair, T. Et, and . Misteli, High mobility of proteins in the mammalian cell nucleus, Nature, vol.404, pp.604-613, 2000.

T. Powers, P. Et, and . Walter, Regulation of Ribosome Biogenesis by the Rapamycin-sensitive TOR-signaling Pathway in Saccharomyces cerevisiae, Molecular Biology of the Cell, vol.10, issue.4, pp.987-1000, 1999.
DOI : 10.1091/mbc.10.4.987

C. Presutti, S. A. Ciafre, and E. I. Bozzoni, The ribosomal protein L2 in S. cerevisiae controls the level of accumulation of its own mRNA, Embo J, vol.10, pp.2215-2236, 1991.

R. H. Reeder, Regulation of RNA Polymerase I Transcription in Yeast and Vertebrates, Prog Nucleic Acid Res Mol Biol, vol.62, pp.293-327, 1999.
DOI : 10.1016/S0079-6603(08)60511-5

J. Retel, R. J. Et, and . Planta, Ribosomal Precursor RNA in Saccharomyces carlsbergensis, European Journal of Biochemistry, vol.20, issue.2, pp.248-58, 1967.
DOI : 10.1016/0022-2836(66)90065-9

G. Rigaut, A. Shevchenko, B. Rutz, M. Wilm, M. Mann et al., A generic protein purification method for protein complex characterization and proteome exploration, Nature Biotechnology, vol.17, issue.10, pp.1030-1032, 1999.
DOI : 10.1038/13732

M. V. Rodnina, T. Daviter, K. Gromadski, and E. W. Wintermeyer, Structural dynamics of ribosomal RNA during decoding on the ribosome, Biochimie, vol.84, issue.8, pp.745-54, 2002.
DOI : 10.1016/S0300-9084(02)01409-8

W. Ross, K. K. Gosink, J. Salomon, K. Igarashi, C. Zou et al., A third recognition element in bacterial promoters: DNA binding by the alpha subunit of RNA polymerase, Science, vol.262, issue.5138, pp.1407-1420, 1993.
DOI : 10.1126/science.8248780

W. Ross, J. F. Thompson, J. T. Newlands, and R. L. Gourse, E.coli Fis protein activates ribosomal RNA transcription in vitro and in vivo, Embo J, vol.9, pp.3733-3775, 1990.
URL : https://hal.archives-ouvertes.fr/in2p3-00008924

M. P. Rout, G. Blobel, and J. D. Aitchison, A Distinct Nuclear Import Pathway Used by Ribosomal Proteins, Cell, vol.89, issue.5, pp.715-740, 1997.
DOI : 10.1016/S0092-8674(00)80254-8

J. J. Sandmeier, S. French, Y. Osheim, W. L. Cheung, C. M. Gallo et al., RPD3 is required for the inactivation of yeast ribosomal DNA genes in stationary phase, The EMBO Journal, vol.21, issue.18, pp.4959-68, 2002.
DOI : 10.1093/emboj/cdf498

F. Saracino, J. Bassler, D. Muzzini, E. Hurt, and M. L. Carbone, The yeast kinase Swe1 is required for proper entry into cell cycle after arrest due to ribosome biogenesis et protein synthesis defects, Cell Cycle, vol.3, pp.648-54, 2004.

A. Sato, G. Kobayashi, H. Hayashi, H. Yoshida, A. Wada et al., The GTP binding protein Obg homolog ObgE is involved in ribosome maturation, Genes to Cells, vol.322, issue.5, pp.393-408, 2005.
DOI : 10.1111/j.1365-2443.2005.00851.x

C. Saveanu, D. Bienvenu, A. Namane, P. E. Gleizes, N. Gas et al., Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps, The EMBO Journal, vol.20, issue.22, pp.6475-84, 2001.
DOI : 10.1093/emboj/20.22.6475
URL : https://hal.archives-ouvertes.fr/pasteur-01404705

C. Saveanu, A. Namane, P. E. Gleizes, A. Lebreton, J. C. Rousselle et al., Sequential Protein Association with Nascent 60S Ribosomal Particles, Sequential Protein Association with Nascent 60S Ribosomal Particles, pp.4449-60, 2003.
DOI : 10.1128/MCB.23.13.4449-4460.2003
URL : https://hal.archives-ouvertes.fr/hal-01350680

A. Scherl, Y. Coute, C. Deon, A. Calle, K. Kindbeiter et al., Functional Proteomic Analysis of Human Nucleolus, Molecular Biology of the Cell, vol.13, issue.11, pp.4100-4109, 2002.
DOI : 10.1091/mbc.E02-05-0271
URL : https://hal.archives-ouvertes.fr/hal-00179628

M. E. Schmitt, D. A. Et, and . Clayton, Nuclear RNase MRP is required for correct processing of pre-5.8S rRNA in Saccharomyces cerevisiae., Molecular and Cellular Biology, vol.13, issue.12, pp.7935-7976, 1993.
DOI : 10.1128/MCB.13.12.7935

B. Senger, D. L. Lafontaine, J. S. Graindorge, O. Gadal, A. Camasses et al., The Nucle(ol)ar Tif6p and Efl1p Are Required for a Late Cytoplasmic Step of Ribosome Synthesis, Molecular Cell, vol.8, issue.6, pp.1363-73, 2001.
DOI : 10.1016/S1097-2765(01)00403-8

M. R. Sharma, C. Barat, D. N. Wilson, T. M. Booth, M. Kawazoe et al., Interaction of Era with the 30S Ribosomal Subunit, Molecular Cell, vol.18, issue.3, pp.319-348, 2005.
DOI : 10.1016/j.molcel.2005.03.028

K. Shuai, J. R. Et, and . Warner, defective in pre-rRNA processing, Nucleic Acids Research, vol.19, issue.18, pp.5059-64, 1991.
DOI : 10.1093/nar/19.18.5059

P. Siekevitz, P. C. Et, and . Zamecnik, In vitro incorporation of l-C14-DL-alanine into proteins of rat liver granular fractions, Fed. Proc, vol.10, pp.246-247, 1951.

J. E. Smith, B. S. Cooperman, and E. P. Mitchell, Methylation sites in Escherichia coli ribosomal RNA: Localization and identification of four new sites of methylation in 23 S rRNA, Biochemistry, vol.31, issue.44, pp.10825-10859, 1992.
DOI : 10.1021/bi00159a025

J. S. Smith, J. D. Et, and . Boeke, An unusual form of transcriptional silencing in yeast ribosomal DNA., Genes & Development, vol.11, issue.2, pp.241-54, 1997.
DOI : 10.1101/gad.11.2.241

J. Spacek, Atlas of ultrastructural neurocytology, 2000.

C. M. Spahn, R. Beckmann, N. Eswar, P. A. Penczek, A. Sali et al., Structure of the 80S Ribosome from Saccharomyces cerevisiae???tRNA-Ribosome and Subunit-Subunit Interactions, Cell, vol.107, issue.3, pp.373-86, 2001.
DOI : 10.1016/S0092-8674(01)00539-6

Y. Sydorskyy, D. J. Dilworth, E. C. Yi, D. R. Goodlett, R. W. Wozniak et al., Intersection of the Kap123p-Mediated Nuclear Import and Ribosome Export Pathways, Molecular and Cellular Biology, vol.23, issue.6, pp.2042-54, 2003.
DOI : 10.1128/MCB.23.6.2042-2054.2003

M. W. Talkington, G. Siuzdak, and J. R. Williamson, An assembly landscape for the 30S ribosomal subunit, Nature, vol.5, issue.7068, pp.628-660, 2005.
DOI : 10.1038/nature04261

A. Tissieres, J. W. Et, and . Hopkins, FACTORS AFFECTING AMINO ACID INCORPORATION INTO PROTEINS BY ESCHERICHIA COLI RIBOSOMES, Proceedings of the National Academy of Sciences, vol.47, issue.12, pp.2015-2038, 1961.
DOI : 10.1073/pnas.47.12.2015

A. Tissieres, J. D. Et, and . Watson, Ribonucleoprotein Particles from Escherichia Coli, Nature, vol.75, issue.4638, pp.778-80, 1958.
DOI : 10.1016/0006-3002(57)90171-3

P. Traub, M. Et, and . Nomura, Structure and function of Escherichia coli ribosomes, Journal of Molecular Biology, vol.40, issue.3, pp.391-413, 1969.
DOI : 10.1016/0022-2836(69)90161-2

S. A. Udem, J. R. Et, and . Warner, The cytoplasmic maturation of a ribosomal precursor ribonucleic acid in yeast, J Biol Chem, vol.248, pp.1412-1418, 1973.

G. Valentin, Repertorium für Anatomie und Physiologie, 1839.

C. A. Van-beekvelt, R. E. Jeeninga, J. Van-'t-riet, J. Venema, and H. A. Raue, Identification of cis-acting elements involved in 3???-end formation of Saccharomyces cerevisiae 18S rRNA, RNA, vol.7, issue.6, pp.896-903, 2001.
DOI : 10.1017/S1355838201010196

A. Van-hoof, P. Lennertz, and R. Parker, Three conserved members of the RNase D family have unique and overlapping functions in the processing of 5S, 5.8S, U4, U5, RNase MRP and RNase P RNAs in yeast, The EMBO Journal, vol.19, issue.6, pp.1357-65, 2000.
DOI : 10.1093/emboj/19.6.1357

E. Vanrobays, J. P. Gelugne, M. Caizergues-ferrer, and E. D. Lafontaine, Dim2p, a KH-domain protein required for small ribosomal subunit synthesis, RNA, vol.10, issue.4, pp.645-56, 2004.
DOI : 10.1261/rna.5162204
URL : https://hal.archives-ouvertes.fr/hal-00022407

I. V. Velichutina, M. J. Rogers, T. F. Mccutchan, and S. W. Liebman, Chimeric rRNAs containing the GTPase centers of the developmentally regulated ribosomal rRNAs of Plasmodium falciparum are functionally distinct, RNA, vol.4, issue.5, pp.594-602, 1998.
DOI : 10.1017/S1355838298980049

J. A. Vetro and Y. H. Chang, Yeast methionine aminopeptidase type 1 is ribosome-associated and requires its N-terminal zinc finger domain for normal function in vivo, Journal of Cellular Biochemistry, vol.246, issue.4, pp.678-88, 2002.
DOI : 10.1002/jcb.10161

T. C. Walther, H. S. Pickersgill, V. C. Cordes, M. W. Goldberg, T. D. Allen et al., The cytoplasmic filaments of the nuclear pore complex are dispensable for selective nuclear protein import, The Journal of Cell Biology, vol.68, issue.1, pp.63-77, 2002.
DOI : 10.1038/376184a0

C. Wang, U. T. Et, and . Meier, Architecture and assembly of mammalian H/ACA small nucleolar and telomerase ribonucleoproteins, The EMBO Journal, vol.19, issue.8, pp.1857-67, 2004.
DOI : 10.1038/sj.emboj.7600181

J. R. Warner, The economics of ribosome biosynthesis in yeast, Trends in Biochemical Sciences, vol.24, issue.11, pp.437-477, 1999.
DOI : 10.1016/S0968-0004(99)01460-7

J. R. Warner, P. M. Knopf, and E. A. Rich, A MULTIPLE RIBOSOMAL STRUCTURE IN PROTEIN SYNTHESIS, Proceedings of the National Academy of Sciences, vol.49, issue.1, pp.122-131, 1963.
DOI : 10.1073/pnas.49.1.122

J. R. Warner, R. Et, and . Soeiro, Nascent ribosomes from HeLa cells., Proceedings of the National Academy of Sciences, vol.58, issue.5, pp.1984-90, 1967.
DOI : 10.1073/pnas.58.5.1984

C. Weitzmann, S. J. Tumminia, M. Boublik, and E. J. Ofengand, C967 by their respective methyltransferases, Nucleic Acids Research, vol.19, issue.25, pp.7089-95, 1991.
DOI : 10.1093/nar/19.25.7089

J. P. Bachellerie, B. Michot, M. Nicoloso, A. Balakin, J. Ni et al., Antisense snoRNAs: a family of nucleolar RNAs with long complementarities to rRNA, Trends in Biochemical Sciences, vol.20, issue.7, pp.261-264, 1995.
DOI : 10.1016/S0968-0004(00)89039-8

N. Ban, P. Nissen, J. Hansen, P. B. Moore, and T. A. Steitz, The Complete Atomic Structure of the Large Ribosomal Subunit at 2.4 A Resolution, Science, vol.289, issue.5481, pp.905-920, 2000.
DOI : 10.1126/science.289.5481.905

J. Bassler, P. Grandi, O. Gadal, T. Lessmann, E. Petfalski et al., Identification of a 60S Preribosomal Particle that Is Closely Linked to Nuclear Export, Molecular Cell, vol.8, issue.3, pp.517-529, 2001.
DOI : 10.1016/S1097-2765(01)00342-2

E. Bertrand, F. Houser-scott, A. Kendall, R. H. Singer, and D. R. Engelke, Nucleolar localization of early tRNA processing, Genes & Development, vol.12, issue.16, pp.2463-2468, 1998.
DOI : 10.1101/gad.12.16.2463

M. C. Daugeron and P. Linder, Dbp7p, a putative ATP-dependent RNA helicase from Saccharomyces cerevisiae, is required for 60S ribosomal subunit assembly, RNA, vol.4, issue.5, pp.566-581, 1998.
DOI : 10.1017/S1355838298980190

F. Dragon, J. E. Gallagher, P. A. Compagnone-post, B. M. Mitchell, K. A. Porwancher et al., A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis, Nature, vol.180, issue.6892, pp.967-970, 2002.
DOI : 10.1073/PNAS.97.24.13027

A. Fatica, A. D. Cronshaw, M. Dlakic, and D. Tollervey, Ssf1p Prevents Premature Processing of an Early Pre-60S Ribosomal Particle, Molecular Cell, vol.9, issue.2, pp.341-351, 2002.
DOI : 10.1016/S1097-2765(02)00458-6

A. Fatica and D. Tollervey, Making ribosomes, Current Opinion in Cell Biology, vol.14, issue.3, pp.313-318, 2002.
DOI : 10.1016/S0955-0674(02)00336-8

A. P. Gasch, P. T. Spellman, C. M. Kao, O. Carmel-harel, M. B. Eisen et al., Genomic Expression Programs in the Response of Yeast Cells to Environmental Changes, Molecular Biology of the Cell, vol.11, issue.12, pp.4241-4257, 2000.
DOI : 10.1091/mbc.11.12.4241

P. Harnpicharnchai, J. Jakovljevic, E. Horsey, T. Miles, J. Roman et al., Composition and Functional Characterization of Yeast 66S Ribosome Assembly Intermediates, Molecular Cell, vol.8, issue.3, pp.505-515, 2001.
DOI : 10.1016/S1097-2765(01)00344-6

J. H. Ho, G. Kallstrom, and A. W. Johnson, Nmd3p Is a Crm1p-Dependent Adapter Protein for Nuclear Export of the Large Ribosomal Subunit, The Journal of Cell Biology, vol.53, issue.5, 2000.
DOI : 10.1016/0092-8674(95)90435-2

C. E. Andersen, A. H. Lyon, A. K. Fox, Y. W. Leung, H. Lam et al., Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry, Nature, vol.415, pp.180-183, 2002.

E. Hurt, S. Hannus, B. Schmelzl, D. Lau, D. Tollervey et al., A Novel In Vivo Assay Reveals Inhibition of Ribosomal Nuclear Export in Ran-Cycle and Nucleoporin Mutants, The Journal of Cell Biology, vol.17, issue.3, pp.389-401, 1999.
DOI : 10.1016/S0065-2660(08)60107-8

A. Kumar, S. Agarwal, J. A. Heyman, S. Matson, M. Heidtman et al., Subcellular localization of the yeast proteome, Genes & Development, vol.16, issue.6, pp.707-719, 2002.
DOI : 10.1101/gad.970902

D. L. Lafontaine and D. Tollervey, Birth of the snoRNPs: the evolution of the modification-guide snoRNAs, Trends in Biochemical Sciences, vol.23, issue.10, pp.383-388, 1998.
DOI : 10.1016/S0968-0004(98)01260-2

M. S. Longtine, A. Mckenzie, I. , D. J. Demarini, N. G. Shah et al., Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae, Yeast, vol.13, issue.10, pp.953-961, 1998.
DOI : 10.1002/(SICI)1097-0061(199807)14:10<953::AID-YEA293>3.0.CO;2-U

T. A. Nissan, J. Bassler, E. Petfalski, D. Tollervey, and E. Hurt, 60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasm, The EMBO Journal, vol.21, issue.20, pp.5539-5547, 2002.
DOI : 10.1093/emboj/cdf547

Y. Ohtake and R. B. Wickner, Yeast virus propagation depends critically on free 60S ribosomal subunit concentration., Molecular and Cellular Biology, vol.15, issue.5, pp.2772-2781, 1995.
DOI : 10.1128/MCB.15.5.2772

H. S. Park, A. Himmelbach, K. S. Browning, T. Hohn, and L. A. Ryabova, A Plant Viral ???Reinitiation??? Factor Interacts with the Host Translational Machinery, Cell, vol.106, issue.6, pp.723-733, 2001.
DOI : 10.1016/S0092-8674(01)00487-1

J. H. Park, B. C. Jensen, C. T. Kifer, and M. Parsons, A novel nucleolar G-protein conserved in eukaryotes, J. Cell Sci, vol.114, pp.173-185, 2001.

J. R. Pringle, A. E. Adams, D. G. Drubin, and B. K. Haarer, [40] Immunofluorescence methods for yeast, Methods Enzymol, vol.194, pp.565-602, 1991.
DOI : 10.1016/0076-6879(91)94043-C

M. P. Rout, J. D. Aitchison, A. Suprapto, K. Hjertaas, Y. Zhao et al., The Yeast Nuclear Pore Complex, The Journal of Cell Biology, vol.17, issue.4, pp.635-651, 2000.
DOI : 10.1016/S1097-2765(00)80023-4

C. Saveanu, D. Bienvenu, A. Namane, P. E. Gleizes, N. Gas et al., Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps, The EMBO Journal, vol.20, issue.22, pp.6475-6484, 2001.
DOI : 10.1093/emboj/20.22.6475
URL : https://hal.archives-ouvertes.fr/pasteur-01404705

C. M. Smith and J. A. Steitz, Sno Storm in the Nucleolus: New Roles for Myriad Small RNPs, Cell, vol.89, issue.5, pp.669-672, 1997.
DOI : 10.1016/S0092-8674(00)80247-0

J. D. Thompson, T. J. Gibson, F. Plewniak, F. Jeanmougin, and D. G. Higgins, The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools, Nucleic Acids Research, vol.25, issue.24, pp.4876-4882, 1997.
DOI : 10.1093/nar/25.24.4876

J. Trapman, J. Retel, and R. J. Planta, Ribosomal precursor particles from yeast, Experimental Cell Research, vol.90, issue.1, pp.95-104, 1975.
DOI : 10.1016/0014-4827(75)90361-4

S. A. Udem and J. R. Warner, The cytoplasmic maturation of a ribosomal precursor ribonucleic acid in yeast, J. Biol. Chem, vol.248, pp.1412-1416, 1973.

J. R. Warner, The assembly of ribosomes in eukaryotes, Ribosomes, pp.461-488, 1974.

K. A. Wehner and S. J. Baserga, The sigma(70)-like motif: a eukaryotic RNA binding domain unique to a superfamily of proteins required for ribosome biogenesis, Mol. Cell MOL. CELL. BIOL, vol.9, pp.329-339, 2002.

R. 1. Warner and J. R. , The economics of ribosome biosynthesis in yeast, Trends in Biochemical Sciences, vol.24, issue.11, pp.437-440, 1999.
DOI : 10.1016/S0968-0004(99)01460-7

M. Dundr and T. Misteli, Functional architecture in the cell nucleus, Biochemical Journal, vol.356, issue.2, pp.297-310, 2001.
DOI : 10.1042/bj3560297

M. Fromont-racine, B. Senger, C. Saveanu, and F. Fasiolo, Ribosome assembly in eukaryotes, Gene, vol.313, pp.17-42, 2003.
DOI : 10.1016/S0378-1119(03)00629-2
URL : https://hal.archives-ouvertes.fr/pasteur-01404699

H. Tschochner and E. Hurt, Pre-ribosomes on the road from the nucleolus to the cytoplasm, Trends in Cell Biology, vol.13, issue.5, pp.255-263, 2003.
DOI : 10.1016/S0962-8924(03)00054-0

Y. Ho, A. Gruhler, A. Heilbut, G. D. Bader, L. Moore et al., Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry, Nature, vol.415, issue.6868, pp.180-183, 2002.
DOI : 10.1038/415180a

N. J. Krogan, W. T. Peng, G. Cagney, M. D. Robinson, R. Haw et al., High-Definition Macromolecular Composition of Yeast RNA-Processing Complexes, Molecular Cell, vol.13, issue.2, pp.225-239, 2004.
DOI : 10.1016/S1097-2765(04)00003-6

G. Kallstrom, J. Hedges, J. , and A. , The Putative GTPases Nog1p and Lsg1p Are Required for 60S Ribosomal Subunit Biogenesis and Are Localized to the Nucleus and Cytoplasm, Respectively, Molecular and Cellular Biology, vol.23, issue.12, pp.4344-4355, 2003.
DOI : 10.1128/MCB.23.12.4344-4355.2003

C. Saveanu, A. Namane, P. E. Gleizes, A. Lebreton, J. C. Rousselle et al., Sequential Protein Association with Nascent 60S Ribosomal Particles, Molecular and Cellular Biology, vol.23, issue.13, pp.4449-4460, 2003.
DOI : 10.1128/MCB.23.13.4449-4460.2003
URL : https://hal.archives-ouvertes.fr/hal-01350680

X. Wu, G. Ivanova, M. Merup, M. Jansson, B. Stellan et al., Molecular Analysis of the Human Chromosome 5q13.3 Region in Patients with Hairy Cell Leukemia and Identification of Tumor Suppressor Gene Candidates, Genomics, vol.60, issue.2, pp.161-171, 1999.
DOI : 10.1006/geno.1999.5911

M. Shehata, J. D. Schwarzmeier, M. Hilgarth, R. Hubmann, M. Duechler et al., TGF-??1 induces bone marrow reticulin fibrosis in hairy cell leukemia, Journal of Clinical Investigation, vol.113, issue.5, pp.676-685, 2004.
DOI : 10.1172/JCI19540

C. Rain, M. Fromont-racine, U. Bassler, J. , P. Grandi et al., Identifi cation of a 60S preribosomal particle that is closely linked to nuclear export, Mol. Cell, vol.8, pp.517-529, 2001.

U. Basu, K. Si, J. R. Warner, and U. Maitra, The Saccharomyces cerevisiae TIF6 Gene Encoding Translation Initiation Factor 6 Is Required for 60S Ribosomal Subunit Biogenesis, Molecular and Cellular Biology, vol.21, issue.5, pp.1453-1462, 2001.
DOI : 10.1128/MCB.21.5.1453-1462.2001

C. B. Brachmann, A. Davies, G. J. Cost, E. Caputo, J. Li et al., Designer deletion strains derived fromSaccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disruption and other applications, Yeast, vol.11, issue.2, pp.115-132, 1998.
DOI : 10.1002/(SICI)1097-0061(19980130)14:2<115::AID-YEA204>3.0.CO;2-2

C. Dez and D. Tollervey, Ribosome synthesis meets the cell cycle, Current Opinion in Microbiology, vol.7, issue.6, pp.631-637, 2004.
DOI : 10.1016/j.mib.2004.10.007

A. Fatica and D. Tollervey, Making ribosomes, Current Opinion in Cell Biology, vol.14, issue.3, pp.313-318, 2002.
DOI : 10.1016/S0955-0674(02)00336-8

M. Fromont-racine, J. C. Rain, and P. Legrain, Building protein-protein networks by two-hybrid mating strategy, Methods Enzymol, vol.350, pp.513-524, 2002.
DOI : 10.1016/S0076-6879(02)50982-4

O. Gadal, B. D. Strau, J. Kessl, B. Trumpower, D. Tollervey et al., Nuclear Export of 60S Ribosomal Subunits Depends on Xpo1p and Requires a Nuclear Export Sequence-Containing Factor, Nmd3p, That Associates with the Large Subunit Protein Rpl10p, Molecular and Cellular Biology, vol.21, issue.10, pp.3405-3415, 2001.
DOI : 10.1128/MCB.21.10.3405-3415.2001

J. Hedges, M. West, and A. W. Johnson, Release of the export adapter, Nmd3p, from the 60S ribosomal subunit requires Rpl10p and the cytoplasmic GTPase Lsg1p, The EMBO Journal, vol.153, issue.3, pp.567-579, 2005.
DOI : 10.1016/S0962-8924(03)00054-0

J. H. Ho, G. Kallstrom, and A. W. Johnson, Nascent 60S ribosomal subunits enter the free pool bound by Nmd3p, RNA, vol.6, issue.11, pp.1625-1634, 2000.
DOI : 10.1017/S1355838200001291

K. Taylor, K. Bennett, and . Boutilier, Systematic identifi cation of protein complexes in Saccharomyces cerevisiae by mass spectrometry, Nature, vol.415, pp.180-183, 2002.

M. Iwase and A. Toh-e, Ybr267w is a New Cytoplasmic Protein Belonging to the Mitotic Signaling Network of Saccharomyces cerevisiae, Cell Structure and Function, vol.29, issue.1, pp.1-15, 2004.
DOI : 10.1247/csf.29.1

A. W. Johnson, E. Lund, and J. Dahlberg, Nuclear export of ribosomal subunits, Trends in Biochemical Sciences, vol.27, issue.11, pp.580-585, 2002.
DOI : 10.1016/S0968-0004(02)02208-9

G. Kallstrom, J. Hedges, and A. Johnson, The Putative GTPases Nog1p and Lsg1p Are Required for 60S Ribosomal Subunit Biogenesis and Are Localized to the Nucleus and Cytoplasm, Respectively, Molecular and Cellular Biology, vol.23, issue.12, pp.4344-4355, 2003.
DOI : 10.1128/MCB.23.12.4344-4355.2003

D. Masson and T. E. Kreis, Identification and molecular characterization of E-MAP-115, a novel microtubule-associated protein predominantly expressed in epithelial cells, The Journal of Cell Biology, vol.123, issue.2, pp.357-371, 1993.
DOI : 10.1083/jcb.123.2.357

T. I. Moy and P. A. Silver, Nuclear export of the small ribosomal subunit requires the Ran-GTPase cycle and certain nucleoporins, Genes & Development, vol.13, issue.16, pp.2118-2133, 1999.
DOI : 10.1101/gad.13.16.2118

J. R. Pringle, A. E. Adams, D. G. Drubin, and B. K. Haarer, [40] Immunofluorescence methods for yeast, Methods Enzymol, vol.194, pp.565-602, 1991.
DOI : 10.1016/0076-6879(91)94043-C

G. Rigaut, A. Shevchenko, B. Rutz, M. Wilm, M. Mann et al., A generic protein purifi cation method for protein complex characterization and proteome exploration, Nature Biotechnology, vol.17, issue.10, pp.1030-1032, 1999.
DOI : 10.1038/13732

M. P. Rout, G. Blobel, J. D. Aitchison, and M. Tyers, A Distinct Nuclear Import Pathway Used by Ribosomal Proteins, Cell, vol.89, issue.5, pp.715-725, 1997.
DOI : 10.1016/S0092-8674(00)80254-8

C. Saveanu, D. Bienvenu, A. Namane, P. E. Gleizes, N. Gas et al., Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps, The EMBO Journal, vol.20, issue.22, pp.6475-6484, 2001.
DOI : 10.1093/emboj/20.22.6475
URL : https://hal.archives-ouvertes.fr/pasteur-01404705

K. Si and U. Maitra, Homologue of Mammalian Translation Initiation Factor 6 Does Not Function as a Translation Initiation Factor, Molecular and Cellular Biology, vol.19, issue.2, pp.1416-1426, 1999.
DOI : 10.1128/MCB.19.2.1416

H. Tschochner and E. Hurt, Pre-ribosomes on the road from the nucleolus to the cytoplasm, Trends in Cell Biology, vol.13, issue.5, pp.255-263, 2003.
DOI : 10.1016/S0962-8924(03)00054-0

D. M. Valenzuela, A. Chaudhuri, and U. Maitra, Eukaryotic ribosomal subunit anti-association activity of calf liver is contained in a single polypeptide chain protein of Mr, p.500, 1982.

C. B. Brachmann, A. Davies, G. J. Cost, E. Caputo, J. Li et al., Designer deletion strains derived fromSaccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disruption and other applications, Yeast, vol.11, issue.2, pp.115-147, 1998.
DOI : 10.1002/(SICI)1097-0061(19980130)14:2<115::AID-YEA204>3.0.CO;2-2

M. Hofert, M. Schelder, H. Brajenovic, A. Ru-ffner, K. Merino et al., Functional organization of the yeast proteome by systematic analysis o f p rotein complexes, Nature, vol.415, pp.141-148, 2002.

S. Ghaemmaghami, W. K. Huh, K. Bower, R. W. Howson, A. Belle et al., Global analysis of protein expression in yeast, Nature, vol.425, issue.6959, pp.737-778, 2003.
DOI : 10.1038/nature02046

G. Rigaut, A. Shevchenko, B. Rutz, M. Wilm, M. Mann et al., A generic protein purification method fo r protein complex characterization and proteome exploration, Nature Biotechnology, vol.17, issue.10, pp.1030-1032, 1999.
DOI : 10.1038/13732

C. Saveanu, D. Bienvenu, A. Namane, P. E. Gleizes, N. Gas et al., Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps, The EMBO Journal, vol.20, issue.22, pp.6475-6484, 2001.
DOI : 10.1093/emboj/20.22.6475
URL : https://hal.archives-ouvertes.fr/pasteur-01404705

C. Travail, L. Matap, G. Matap, G. , N. Matap et al., RLP24?TAP:TRP1, rei1?::KanMX4 Ce travail LMA333 MATa, trp1-289, ura3-52, ade2, leu2,-3-112, arg4, RPL24B-TAP:TRP1 Ce travail LMA334 MATaKanMX6 Ce travail LMA378 MAT? ura3?0, his3?1, leu2?0, lys2?0, rpl24a?::NAT Ce travail LMA379 MATa, ura3?0, his3?1, leu2?0, met15?0, rpl24b?::HYG Ce travail LMA380 MATa, ura3?0, his3?1, leu2?0, lys2?0, met15?0, rpl24a?::NAT, rpl24b?::HYG Ce travail LMA387 MATa, ura3?0, his3?1, leu2?0, met15?0, REI1-GFP(S65T):His3MX6 Ce travail LMA389 MATa, ura3?0, his3?1, leu2?0, met15?0, ARX1-TAP:His3MX6 Ce travail LMA392 MATa, ura3?0, his3?1, leu2?0, lys2?0, met15?0, rpl24a?::NAT, rpl24b?::HYG, REI1?TAP:URA3 Ce travail LMA393 MAT?, ura3?0, his3?1, leu2?0, lys2?0, met15?0, REI1-TAP:URA3 Ce travail LMA394 MATa, ura3?0, his3?1, leu2?0, met15?0, REI1-GFP(S65T):His3MX6 Ce travail LMA397 MATa, ura3?0, his3?1, leu2?0, met15?0, ARX1-TAP:His3MX6 Ce travail LMA399 MATa, ura3?0, his3?1, leu2?0, met15?0, ARX1-TAP:His3MX6, rei1?::KanMX4 Ce travail LMA401 MATa, ura3?0, his3?1, leu2?0, met15?0, ARX1-GFP(S65T):His3MX6 Ce travail LMA402 MAT?, ura3?0, his3?1, leu2?0, lys2?0, KanMX6:P GAL1 -NOG1, NSA2-TAP:His3MX6 Ce travail::NAT Ce travail LMA535-B MAT?, ura3?0, his3?1, leu2?0, lys2?, met15?, arx1?::KanMX4 Ce travail LMA536-C MATa, ura3?0, his3?1, leu2?0, lys2?, met15?, RLP24? TAP:TRP1, REI1?13Myc:KanMX6 Ce travail LMA373 MATaS65T):His3MX6, arx1?::KanMX4 Ce travailS65T):His3MX6, rei1?::KanMX4 Ce travail LMA432 MAT?NAT, rpl24b?::HYG, rei1?::KanMX4 Ce travail LMA472 MATa rei1?::NAT, RLP24-TAP:TRP1 Ce travail LMA479 MATaNAT, KanMX6:P GAL1 -REH1 Ce travail LMA510 MATa:P GAL1 -KAP121, ALB1-GFP(S65T):His3MX6 Ce travail LMA544-B MATa, ura3?0, his3?1, leu2?0, lys2?, met15?, arx1?::KanMX4, ALB1-GFP(S65T):His3MX6 Ce travail LMA544-C MATa, ura3?0, his3?1, leu2?0, lys2?, met15?, arx1?::KanMX4 Ce travail Souche Génotype Source LMA545-A MATaS65T):His3MX6 Ce travail LMA576 MATa KanMX6:P GAL1 -TIF6 Ce travail complémentation, nous souhaitions disposer de plasmides permettant l'utilisation de la stratégie Gateway® En amont de cette cassette, j'ai introduit la séquence 5' non transcrite du gène de RLP24, de sorte à placer les gènes à exprimer sous contrôle d'un promoteur préribosomique (Figure 60). L'ensemble du site de clonage a par la suite été transféré dans pFL38 (centromérique) et pFL44 (multicopie) portant le marqueur URA3. 46 Pfo I 179 Apa BI 179 Bst API 183 Nde I 716 Apa I 716 Psp OMI 1487 Nco I 1719 Bst Z17I, pp.1-289, 1910.

. Bae, Bst EII 3866 Age I 3884 Psr I 3931 Afl I I 4166 Xcm I final comprenait la séquence du gène à cloner avec des sites de restriction adéquats. Pour pAL66-h (Figure 61), le produit de PCR recouvrait la séquence totale du plasmide père pAL66, en intégrant TINP1 à la place de NSA2. 46 Pfo I 183 Nde I 306 Pvu I I 402 Ban I I 402 Eco ICRI 402 Sac I 412 Sma I 412 Xma I 417 Bam HI 457 Bsp EI 736 Blp I 1218 Bst Z17I 1516 Sbf I 1517 Pst I 1523 Sph I 1535 Not I 1536 Eag I 1544 Pac I 1554 Asc, I 3417 Sac I I 3504 Ppu MI 3504 Sse 8647I 3650