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Theses

Les colloïdes magnétiques et leur utilisation
biophysique dans la détection, le guidage et le
suivi cellulaire in vitro et in vivo.

Abstract : The colloïds used are composed of magnetic nanoparticles (10 nm iron oxide nanocrystals) dispersed in aqueous suspension, and bearing net negative surface charge. Those nanoparticles adsorb through electrostatic interactions on the membrane of most cell types, and are then spontaneously internalised inside intracellular vesicles. It becomes thus possible to magnetically labeled cells. This magnetic labeling can be used to non-invasively follow-up by MRI the fate of therapeutic cells implanted inside the organism. Such labeling has been used to monitor the fate of rat smooth muscle cells in an abdominal aortic aneurysm model and of muscular explants in a urinary incontinence model. In each case, modification of MRI contrast properties over time has been investigated both in vitro and in vivo. Cell magnetic properties have also been used to apply external forces and investigate their influence on cell migration in vitro as well as their potential use for in vivo cell targeting.
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https://tel.archives-ouvertes.fr/tel-00096699
Contributor : Charlotte Riviere <>
Submitted on : Tuesday, September 19, 2006 - 9:43:49 PM
Last modification on : Wednesday, December 9, 2020 - 3:12:37 PM

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  • HAL Id : tel-00096699, version 1

Citation

Charlotte Riviere. Les colloïdes magnétiques et leur utilisation
biophysique dans la détection, le guidage et le
suivi cellulaire in vitro et in vivo.. Biophysique [physics.bio-ph]. Université Pierre et Marie Curie - Paris VI, 2005. Français. ⟨tel-00096699⟩

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