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Cinétique de détachement de microorganismes modèles adsorbés sur des surfaces d'acier inoxydable : effet de la rugosité et de l'orientation cristallographique

Abstract : Bioadhesion of micro-organisms on the stainless steels are commoplace in food industry, catering and appliances and can involve problems of publics health such as toxicological infections. In partnership with Ugine-Alz, we studied, using a radial flow chamber (Décavé et al. Biophysical Journal, 2002, 82, 2383-95), the kinetics of detachment of three micro-organims after adhesion on stainless steel surfaces. Saccharomyces cerevisiae (yeast), Escherichia coli (Gram-), and Staphylococcus epidermidis (Gram+). The stainless steels used are polished mirror and etched samples with different grain size and depths of attack of grain boundaries. The use of these various surface qualities does not modify teh hydrodynamic value of the shear stress necessary to detach 50% of the micro-organisms (threshold of detachment). On the other hand, the depth of the grain boundaries and the size of grain accelerate the kinetics of detachment of S. cerevisiae and slow down that of E. coli. Lastly, a preferential adhesion was noted, for the three types of cells, on the grains of crystallographic orientation <001> (Demilly et al., Colloids and Surfaces B, in press, 2006).
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https://tel.archives-ouvertes.fr/tel-00095379
Contributor : Magali Demilly <>
Submitted on : Friday, September 15, 2006 - 3:36:48 PM
Last modification on : Friday, November 6, 2020 - 3:50:47 AM
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Magali Demilly. Cinétique de détachement de microorganismes modèles adsorbés sur des surfaces d'acier inoxydable : effet de la rugosité et de l'orientation cristallographique. Biophysique [physics.bio-ph]. Université Joseph-Fourier - Grenoble I, 2006. Français. ⟨tel-00095379⟩

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