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MISE AU POINT D'UN FORMAT DE PUCES A CELLULES POUR L'ANALYSE PHENOTYPIQUE A HAUT-CONTENU

Abstract : Nowadays biology research is faced with a considerable amount of genomic data which has to be functionally characterised in order to bring new concepts, which will lead to new therapies. These studies approach molecular-based mechanisms, and need high-throughput and parallel data management. The multidisciplinary work presented here has allowed the development of a miniaturised technology of cell culture in liquid drops, matrixed on a plane solid substrate, to test the action of molecules or conditions on the cells behaviour.
After the macroscopic demonstration of nucleic acid molecule transfection in living cells on such a device, we have developed a fabrication protocol for a miniaturised support, able to maintain 100 nano-drops per cm², based on differential surface tensions. A picoliter dispensing robot was integrated to make the cell culture drops automatically. The cells' behaviour in the drops is analysed by high-content fluorescence microscopy after fixation. Each cell of each drop is characterised by tenths of parameters individually.
This new analytical approach, which has triggered the development of new bio-statistical tools, has been applied to a multipartner project of siRNA screening, aimed at studying the impact of genes on glioblastoma cells chemoresistance. We have also shown the use of mass spectrometry as a multiparametric phenotyping method. This cell-on-chip technology seems particularly well suited for the study of the precise behaviour of cells among a population at high-throughput. It will be the base of new lab-on-chip technologies.
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https://tel.archives-ouvertes.fr/tel-00092909
Contributor : Julien Reboud <>
Submitted on : Tuesday, September 12, 2006 - 3:03:18 PM
Last modification on : Friday, November 6, 2020 - 3:52:32 AM
Long-term archiving on: : Monday, April 5, 2010 - 11:37:56 PM

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  • HAL Id : tel-00092909, version 1

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Julien Reboud. MISE AU POINT D'UN FORMAT DE PUCES A CELLULES POUR L'ANALYSE PHENOTYPIQUE A HAUT-CONTENU. Sciences du Vivant [q-bio]. Université Joseph-Fourier - Grenoble I, 2006. Français. ⟨tel-00092909⟩

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