Skip to Main content Skip to Navigation
Theses

Analyse des mouvements des granules de sécrétion à proximité de la membrane plasmique par microscopie de fluorescence à excitation par onde évanescente

Abstract : Regulated hormone secretion is a multi-step process. Secretory granules (SG), which contain hormones, are formed by budding from the trans-golgi network and then migrate towards the cell periphery. Hormones are released by exocytosis when cells are stimulated. We applied total internal reflection fluorescence microscopy to study the dynamics of SG located in the subplasmalemmal region. We developed a motion analysis method to highlight transient behaviours along three-dimensional SG trajectories. The application of this method to the study of the effects of cytoskeletal drugs on SG dynamics and double labelling observations allowed us to associate each type of movement with a particular environment (SG linked to the plasma membrane, the actin filaments or the microtubules). We also studied the role of the complex formed by the proteins Rab27A, MyRIP and Myosin Va during the capture of SG in the cell periphery and their attachment to actin filaments.
Complete list of metadata

https://tel.archives-ouvertes.fr/tel-00090014
Contributor : Sébastien Huet <>
Submitted on : Friday, August 25, 2006 - 4:21:27 PM
Last modification on : Thursday, December 10, 2020 - 10:52:45 AM
Long-term archiving on: : Monday, April 5, 2010 - 10:27:45 PM

Identifiers

  • HAL Id : tel-00090014, version 1

Citation

Sébastien Huet. Analyse des mouvements des granules de sécrétion à proximité de la membrane plasmique par microscopie de fluorescence à excitation par onde évanescente. Biophysique [physics.bio-ph]. Université Pierre et Marie Curie - Paris VI, 2006. Français. ⟨tel-00090014⟩

Share

Metrics

Record views

912

Files downloads

2708