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Participation à l'étude de la régulation transcriptionnelle des gènes de Plasmodium falciparum lors du cycle érythrocytaire. Caractérisation de facteurs nucléaires des familles Myb et HMGB.

Abstract : The erythrocytic cycle of Plasmodium falciparum, the causal agent of malaria, alternates between active proliferation and sexual differentiation implying a fine control of gene expression. However, the actors of transcriptional regulation are yet poorly described.
Comparative study was conducted on transcriptomes of parasite clone 3D7 and its gametocyteless derivative F12 using a thematic DNA microarray
and allowed the identification of several differentially expressed genes potentially implicated in regulation of the crucial events of parasite life cycle.
The annotation of transcription factors and regulatory elements led us to analyse a DNA sequence-specific nuclear factor of the tryptophan cluster Myb family. The PfMyb1 protein contained in the nuclear extracts of the parasite is able to bind "Myb Regulatory Element" sequences identified in Plasmodium promoters. Inactivation of pfmyb1 gene using double-stranded RNA showed that this factor is essential for parasite survival and participates in transcriptional regulation of several genes. Then, two DNA structure-specific factors of the "High Mobility Group" HMGB family containing an "HMG-box" domain were characterized by their architectural properties essential for chromatin remodelling. Differences in the activities, localizations, and levels of expression of PfHMGB1 and PfHMGB2 during erythrocytic development underline their nonredundant roles in the parasite.
The characterization of these dynamic DNA/proteins interactions might provide novel strategies to combat this important human pathogen.
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https://tel.archives-ouvertes.fr/tel-00086444
Contributor : Sylvie Briquet <>
Submitted on : Tuesday, July 18, 2006 - 4:35:42 PM
Last modification on : Thursday, December 10, 2020 - 10:51:54 AM
Long-term archiving on: : Tuesday, September 18, 2012 - 4:20:21 PM

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  • HAL Id : tel-00086444, version 1

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Sylvie Briquet. Participation à l'étude de la régulation transcriptionnelle des gènes de Plasmodium falciparum lors du cycle érythrocytaire. Caractérisation de facteurs nucléaires des familles Myb et HMGB.. Biochimie [q-bio.BM]. Université de Versailles-Saint Quentin en Yvelines, 2006. Français. ⟨tel-00086444⟩

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