Skip to Main content Skip to Navigation
Theses

contrôle de la polarité des cellules adhérentes

Abstract : we used fibronectin micro-patterns imposing cells to spread upon adhesive and non-adhesive areas. The relative positioning of these areas was used to impose asymmetric adhesive environment to cells.
Therefore we showed that cells develop large stress fibres upon non-adhesive areas and protrusions upon adhesive ones. This polarity of the actin cytoskeleton induced a specific recruitment of APC, a molecules interacting with both actin and microtubules. Thereby the polarity of the actin network was transmitted to the microtubules which keep growing along stress fibres whereas they stop when contacting adhesive edges. The internal polarity of cells as revealed by the relative positioning of nucleus and centrosome was thus harmonised with the geometry of the extracellular matrix. Furthermore, we showed that this geometry impinge on spindle orientation and cell division axis independently of cell shape.
Complete list of metadatas

https://tel.archives-ouvertes.fr/tel-00012058
Contributor : Manuel Théry <>
Submitted on : Wednesday, March 29, 2006 - 2:51:37 PM
Last modification on : Tuesday, July 21, 2020 - 3:58:56 AM
Long-term archiving on: : Saturday, April 3, 2010 - 9:00:31 PM

Identifiers

  • HAL Id : tel-00012058, version 1

Citation

Manuel Théry. contrôle de la polarité des cellules adhérentes. Biophysique [physics.bio-ph]. Université Paris-Diderot - Paris VII, 2006. Français. ⟨tel-00012058⟩

Share

Metrics

Record views

527

Files downloads

1727