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contrôle de la polarité des cellules adhérentes

Abstract : we used fibronectin micro-patterns imposing cells to spread upon adhesive and non-adhesive areas. The relative positioning of these areas was used to impose asymmetric adhesive environment to cells.
Therefore we showed that cells develop large stress fibres upon non-adhesive areas and protrusions upon adhesive ones. This polarity of the actin cytoskeleton induced a specific recruitment of APC, a molecules interacting with both actin and microtubules. Thereby the polarity of the actin network was transmitted to the microtubules which keep growing along stress fibres whereas they stop when contacting adhesive edges. The internal polarity of cells as revealed by the relative positioning of nucleus and centrosome was thus harmonised with the geometry of the extracellular matrix. Furthermore, we showed that this geometry impinge on spindle orientation and cell division axis independently of cell shape.
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Contributor : Manuel Théry <>
Submitted on : Wednesday, March 29, 2006 - 2:51:37 PM
Last modification on : Thursday, December 10, 2020 - 10:59:19 AM
Long-term archiving on: : Saturday, April 3, 2010 - 9:00:31 PM


  • HAL Id : tel-00012058, version 1


Manuel Théry. contrôle de la polarité des cellules adhérentes. Biophysique []. Université Paris-Diderot - Paris VII, 2006. Français. ⟨tel-00012058⟩



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