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Altérations mitochondriales et stress oxydant pulmonaire en réponse à l'ozone : Effets de l'âge et d'une supplémentation en oméga-3

Abstract : Ozone (O3) is one of the molecular species most reactive to which are exposed living species. O3 acts primarily on the pulmonary system by undicucing oxidative stress. Because susceptibility to oxidative stress varies with age, we studied alterations of pulmonary balance between production of reactive oxygen species (ROS) and their elimination, in immature (21 days), adult (6 months) and old rats (20 months) during O3 exposure (0,5 ppm, 12 h/day for 7 days). For this purpose we have specifically studied pulmonary mitochondria as ROS source, main antioxidant enzyme activities, contents in stress protein (HSP72), 8-oxodGuo and DNA adducts resulting from lipid peroxidation. These works have shown that our protocol of O3 exposure did not induce lung oxidative stress in adult rats. We confirmed that immature and old rats were more sensitive during O3 challenge than adults. Indeed, O3 generates oxidative stress which leads to modification of ventilatory function and pulmonary DNA oxidation in these two populations. Parameters which take part in greatest susceptibility to O3 differ according to the age. We concluded that the mitochondria is not a major source of pulmonary ROS in our model of O3 exposure. Secondly, with the sights of anti-inflammatory properties of polyunsaturated fatty acids Ω3, we studied the effect of a Ω3 supplementation in immature and old rats exposed to O3. The supplementation in Ω3 limits the pulmonary DNA oxidation in immature and old rats. Paradoxically, in old rats this supplementation provokes an increase in lipid peroxidation susceptibility
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Submitted on : Friday, March 24, 2006 - 2:53:01 PM
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Stéphane Servais. Altérations mitochondriales et stress oxydant pulmonaire en réponse à l'ozone : Effets de l'âge et d'une supplémentation en oméga-3. Physiologie [q-bio.TO]. Université Claude Bernard - Lyon I, 2004. Français. ⟨tel-00012031⟩

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