Skip to Main content Skip to Navigation
Theses

Caractérisation électrophysiologique in situ à l'aide de la technique de patch-clamp de la cellule musculaire striée du Nematode Caenorhabditis elegans

Abstract : Caenorhabditis elegans is a powerful model system widely used to investigate the relationships between genes and behaviours. However, physiological studies at the cellular level have been greatly restricted by the small size of the animal. Using in situ patch-clamp and Ca2+ imaging techniques on body-wall muscle cells, we have characterised the main ionic conductances of these cells. We gave evidence for the crucial role of EGL-19 voltage-dependent Ca2+ channels in excitation-contraction coupling and described the activation properties of voltage-dependent and Ca2+-activated K+ channels. We also demonstrated that the UNC-105 Na+ channel of the degenerin family was not mechanically-gated contrary to the genetic-based model, while another member of this channel family was found to be activated by external protons
Complete list of metadata

https://tel.archives-ouvertes.fr/tel-00012028
Contributor : Frédérique Cohen-Adad <>
Submitted on : Friday, March 24, 2006 - 12:11:59 PM
Last modification on : Wednesday, November 20, 2019 - 3:02:41 AM
Long-term archiving on: : Saturday, April 3, 2010 - 10:04:15 PM

Identifiers

  • HAL Id : tel-00012028, version 1

Collections

Citation

Maëlle Jospin. Caractérisation électrophysiologique in situ à l'aide de la technique de patch-clamp de la cellule musculaire striée du Nematode Caenorhabditis elegans. Physiologie [q-bio.TO]. Université Claude Bernard - Lyon I, 2004. Français. ⟨tel-00012028⟩

Share

Metrics

Record views

229

Files downloads

2422