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Caractérisation fonctionnelle de canaux chlorure de la famille des ClC

Abstract : Chloride channels are involved in several processes essential for cellular life, such as membrane potential stabilisation, regulation of cell volume or pH, as well as in transepithelial transport of salt. Since a dozen years, the application of molecular biology to the study of chloride channels permitted the identification of several families of genes. The functional variety of chloride channels may be explained by their molecular diversity. At present, determining the physiological roles played by the cloned chloride channels constitutes a major stake of their study.

Recently, a homology cloning strategy permitted us the isolation of two novel members of the voltage-dependent ClC chloride channel family: xClC-5 and xClC-3. The functional characterization and the determination of the tissular and cellular localization of these proteins were the targets of our study. In this aim, we used the Xenopus oocyte and the HEK 293 cell line as protein expression systems. Using the voltage-clamp technique with double micro-electrodes, we characterized xClC-5 from the electrophysiological point of view and determined its sensitivity to pH, transition metal ions and tyrosine kinase inhibitors. With the use of antibodies, we studied protein glycosylation and examined their tissular and cellular localisation. Our results are discussed in comparison with the results of other groups, paying particular attention to the possible functional models that are now emerging for the ClC-5 and ClC-3 proteins.
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Contributor : Sandra Schmieder <>
Submitted on : Wednesday, March 22, 2006 - 1:07:13 PM
Last modification on : Wednesday, October 14, 2020 - 4:23:52 AM
Long-term archiving on: : Monday, September 17, 2012 - 12:50:40 PM


  • HAL Id : tel-00012007, version 1



Sandra Schmieder. Caractérisation fonctionnelle de canaux chlorure de la famille des ClC. Physiologie [q-bio.TO]. Université Nice Sophia Antipolis, 2000. Français. ⟨tel-00012007⟩



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