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Régulation du couplage excitation-contraction par le cholestérol et l'oxyde nitrique dans la fibre musculaire squelettique de souris

Abstract : Excitation-contraction (EC) coupling of skeletal muscle depends upon interactions between the membrane voltage-sensor (dihydropyridine receptor, DHPR), and the sarcoplasmic reticulum calcium release channel (ryanodine receptor, RyR). The DHPR stands within two membrane structure enriched in cholesterol, the transverse tubule system and the caveolae. Caveolae also contain the nitric oxide (NO) synthase. The present work provides new insights into the functional modulation of EC coupling by cholesterol and NO, using electrophysiological techniques and fluorescence measurements on isolated muscle cells. The membrane cholesterol content regulates the voltage-sensing and calcium channel function of the DHPR. NO specifically affects the RyR function. At physiological levels, NO modulates the channel activation by membrane depolarization; excess NO maintains some RyR within an activated state
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https://tel.archives-ouvertes.fr/tel-00011998
Contributor : Frédérique Cohen-Adad <>
Submitted on : Tuesday, March 21, 2006 - 2:14:23 PM
Last modification on : Wednesday, November 20, 2019 - 3:02:47 AM
Long-term archiving on: : Saturday, April 3, 2010 - 9:19:17 PM

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  • HAL Id : tel-00011998, version 1

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Sandrine Pouvreau. Régulation du couplage excitation-contraction par le cholestérol et l'oxyde nitrique dans la fibre musculaire squelettique de souris. Physiologie [q-bio.TO]. Université Claude Bernard - Lyon I, 2005. Français. ⟨tel-00011998⟩

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