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Caractérisation d'une nouvelle famille de protéines susceptibles d'interagir avec les NEP plastidiales

Abstract : The phage-like RNA polymerases, encoded in thenucleus (NEP ; Nuclear Encoded RNA Polymerase) ensure partially plastidial genome transcription in higher plants. This work underlined the existence of a new protein family potentially able to interact with NEP in dicotyledon plants: NIP proteins (NEP Interacting Protein). NIP proteins are only present in higher plants and their synthesis is light dependant. Their C-terminal region presents a RING finger protein-protein interacting domain. Using immundetection, we show the first time that NIP proteins are integrated into thylakoid membranes, keeping probably NEP close to the membrane on the stroma side. This association to membranes offers new insights into NEP activity in chloroplasts of dicotyledon plants.
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https://tel.archives-ouvertes.fr/tel-00011722
Contributor : Jacinthe Armanda Azevedo <>
Submitted on : Friday, March 3, 2006 - 11:41:01 AM
Last modification on : Friday, November 6, 2020 - 4:07:13 AM
Long-term archiving on: : Tuesday, April 6, 2010 - 4:46:49 PM

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  • HAL Id : tel-00011722, version 1

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Jacinthe Armanda Azevedo. Caractérisation d'une nouvelle famille de protéines susceptibles d'interagir avec les NEP plastidiales. Biochimie [q-bio.BM]. Université Joseph-Fourier - Grenoble I, 2005. Français. ⟨tel-00011722⟩

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