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Etude moléculaire de la bactérie intracellulaire féminisante Wolbachia chez Armadillidium vulgare (crustacé isopode terrestre)

Abstract : Wolbachia is an intracellular Gram(-) bacterium symbiont of many arthropods. In A. vulgare, it induces male feminization (wVul strain). We have characterized the wVul chromosome (circular DNA of 1.75 Mb) that atypically comprises several rrn operon repeats and revealed that restriction profiles are different from those of other characterized Wolbachia strains. Bacterial DNA purification allowed to initiate the sequencing of this genome. A type IV secretion system characterized by two vir operons was highlighted. Expression of these genes in oocytes and analysis of Vir proteins revealed that this system could be functional. Bidimensional maps of proteins from infected and uninfected tissues showed differential expression of proteins involved in host cytoskeletal structure or metabolism and of a RNA helicase, that could interact with host sex determination.
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Submitted on : Monday, March 6, 2006 - 8:50:26 AM
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Christine Felix. Etude moléculaire de la bactérie intracellulaire féminisante Wolbachia chez Armadillidium vulgare (crustacé isopode terrestre). Interactions entre organismes. Université de Poitiers, 2004. Français. ⟨tel-00011718v2⟩

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