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Etude structurale des protéines et des acides nucléiques par RMN. Etude de la répression du gène de la beta-lactamase chez B. licheniformis 749/I. Augmentation de la résolution des spectres RMN multidimensionnels par filtrage Hadamard.

Abstract : Liquid state NMR is a method of choice for the determination of three-dimensional structure of proteins and nucleic acids. The size of molecules that can be studied nowadays with NMR is however limited. In the first part of this work, the structure of the beta-lactamase repressor BlaI of B. licheniformis 749/I and its interaction with DNA have been studied with standard NMR methods. These results provide new insights about the mechanisms of genes repression involved in bacterial resistance to antibiotics, including the methicillin resistance observed for the highly pathogen S. aureus strain. The second part of this manuscript concerns the implementation of two new Hadamard filters that enhance the resolution of spectra in certain multidimensional NMR experiments. These filters allow to separate the correlation peaks of proteins and nucleic acids according to the amino-acid type and to the base type, respectively. This opens the way for the study of larger biological macromolecules by NMR.
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https://tel.archives-ouvertes.fr/tel-00011563
Contributor : Hélène van Melckebeke <>
Submitted on : Wednesday, February 8, 2006 - 2:03:35 PM
Last modification on : Tuesday, October 6, 2020 - 4:12:03 PM
Long-term archiving on: : Saturday, April 3, 2010 - 7:47:05 PM

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  • HAL Id : tel-00011563, version 1

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Hélène van Melckebeke. Etude structurale des protéines et des acides nucléiques par RMN. Etude de la répression du gène de la beta-lactamase chez B. licheniformis 749/I. Augmentation de la résolution des spectres RMN multidimensionnels par filtrage Hadamard.. Sciences pharmaceutiques. Université Joseph-Fourier - Grenoble I, 2005. Français. ⟨tel-00011563⟩

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