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IDENTIFICATION ET CARACTÉRISATION DES CIBLES DE DSP1
CHEZ DROSOPHILA MELANOGASTER.

Abstract : The Polycomb (PcG) and Trithorax (TrxG) proteins maintain the memory of preestablished
transcription patterns by remodeling chromatin structure. In Drosophila, PcG and
TrxG complexes maintain expression of their target genes via fixation to DNA sequences
termed cellular memory modules (CMMs). What are the target genes of the PcG and TrxG
proteins? What are the mechanisms of recruitment of these proteins on CMMs? We tried to
respond to these points. DSP1 (Dorsal Switch Protein 1) is a Drosophila HMGB (High
mobility group)-like protein, which acts as a TrxG or PcG protein depending on the locus
considered. In the present study, we attempted to characterize DSP1 targets genes and
functions in cellular memory. Using chromatin immunoprecipitation experiments (ChIP), we
identify several DSP1 target genes. Here we show that DSP1 binds to a sequence present
within the regulatory region of the Abdominal-B gene, termed Fab-7. We report, using a
transgenic line for Fab-7 regulatory element, that DSP1 binding is essential for CMM
activity. We show for the first time that binding of DSP1 to Fab-7 CMM is important for the
recruitment of PcG proteins. Moreover, we report that DSP1 acts as a TrxG protein in
expression maintenance of the homoeotic gene Sex combs reduced. In this case, DSP1 is
essential during larval development. Lastly, we show that DSP1 is also involved in the
regulation of the knirps gap gene and discuss of its function in initiation of knirps expression.
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https://tel.archives-ouvertes.fr/tel-00011521
Contributor : Patricia Leloup <>
Submitted on : Wednesday, February 1, 2006 - 3:47:58 PM
Last modification on : Thursday, March 5, 2020 - 6:48:49 PM
Long-term archiving on: : Tuesday, April 6, 2010 - 4:41:36 PM

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  • HAL Id : tel-00011521, version 1

Citation

Aurélien Rappailles. IDENTIFICATION ET CARACTÉRISATION DES CIBLES DE DSP1
CHEZ DROSOPHILA MELANOGASTER.. domain_other. Université d'Orléans, 2005. Français. ⟨tel-00011521⟩

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